Allele Dmel\Adar^3.4.Scer\UAS

General Information
Symbol	Dmel\Adar^3.4.Scer\UAS	Species	D. melanogaster
Name		FlyBase ID	FBal0191441
Feature type	allele	Associated gene	Dmel\Adar

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Keegan et al., 2005)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS sequences, fused to a truncated hsp70 promoter, drives expression of cDNA encoding the Adar 3/4 isoform, tagged with T:Zzzz\FLAG and T:Zzzz\His6. (Keegan et al., 2005)
Carried in construct	P{UAS-Adar.3.4} (Keegan et al., 2005, Keegan et al., 2011, Bhogal et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	partially lethal - majority die \| male, with Adar^1F4, Scer\GAL4^Act5C.PI (Keegan et al., 2005) viable, with Scer\GAL4^Mef2.PR (Keegan et al., 2005)
Phenotype Manifest In

Detailed Description
	Statement Reference Among male progeny of rescue crosses that carry both the Adar^1F4 mutation and the Adar^3.4.Scer\UAS construct, the number of Scer\GAL4^Act5C.PI driver flies obtained, expressed as a percentage of the number of control flies lacking a GAL4 driver, is 20%. (Keegan et al., 2005)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	Scer\GAL4^Cha.7.4/Adar^3.4.Scer\UAS rescues Adar^1F4 (Keegan et al., 2011) Scer\GAL4^Cha.7.4/Adar^3.4.Scer\UAS rescues Adar^5G1 (Keegan et al., 2011) Scer\GAL4^elav-C155/Adar^3.4.Scer\UAS rescues Adar^5G1 (Bhogal et al., 2011) Scer\GAL4^scrt-11-6/Adar^3.4.Scer\UAS rescues Adar^5G1 (Bhogal et al., 2011) Scer\GAL4^βtub.PU/Adar^3.4.Scer\UAS rescues Adar^5G1 (Bhogal et al., 2011)
Partially rescues	Scer\GAL4^Act5C.PI, Scer\GAL4^Act5C.PI, Adar^3.4.Scer\UAS partially rescues Adar^1F4 (Keegan et al., 2005)
Fails to rescue	Scer\GAL4^G14/Adar^3.4.Scer\UAS fails to rescue Adar^5G1 (Bhogal et al., 2011) Scer\GAL4^Mhc.PU/Adar^3.4.Scer\UAS fails to rescue Adar^5G1 (Bhogal et al., 2011)
Comments	Ubiquitous expression of Adar[3.4.Scer\UAS] under the control of Scer\GAL4[βtub.PU] completely rescues the neuromuscular junction phenotype observed in Adar[5G1] larvae with respect to synaptic bouton number and branching. (Bhogal et al., 2011) Expression of Adar[3.4.Scer\UAS] under the control of Scer\GAL4[Cha.7.4] rescues the locomotor defects seen in Adar[5G1] and in Adar[1F4] adults. Expression of Adar[3.4.Scer\UAS] under the control of Scer\GAL4[Cha.7.4] significantly rescues the neurodegeneration phenotype seen in the mushroom body calyces and retina of 30 day old Adar[5G1] adults. (Keegan et al., 2011) Expression of Adar^3.4.Scer\UAS, under the control of Scer\GAL4^Act5C.PI, in a Adar^1F4 background partially rescues the Adar^1F4 mutant phenotype. (Keegan et al., 2005)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Adar^3.4.Scer\UAS
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Bhogal et al., 2011, Nat. Neurosci. 14(12): 1517--1524 Modulation of dADAR-dependent RNA editing by the Drosophila fragile X mental retardation protein. [FBrf0216747] Keegan et al., 2011, Nucleic Acids Res. 39(16): 7249--7262 Functional conservation in human and Drosophila of Metazoan ADAR2 involved in RNA editing: loss of ADAR1 in insects. [FBrf0215032] Keegan et al., 2005, EMBO J. 24(12): 2183--2193 Tuning of RNA editing by ADAR is required in Drosophila. [FBrf0187510]

Allele Dmel\Adar3.4.Scer\UAS

Allele Dmel\Adar^3.4.Scer\UAS