FlyBase Allele Report: Dmel\Fs[GD15843]

General Information

Symbol

Dmel\Fs^GD15843

Species

D. melanogaster

Name

FlyBase ID

FBal0207050

Feature type

allele

Associated gene

Dmel\Fs

Associated Insertion(s)

Carried in Construct

P{GD15843}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD15843}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD15843

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number | progressive | spermatogenesis, with Scer\GAL4^NP1624

(Herrera et al., 2021)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

cyst progenitor cell | absent, with Scer\GAL4^NP1624

(Herrera et al., 2021)

hub cell | absent, with Scer\GAL4^NP1624

(Herrera et al., 2021)

hub cell | decreased number, with Scer\GAL4^NP1624

(Herrera et al., 2021)

male germline stem cell | absent, with Scer\GAL4^NP1624

(Herrera et al., 2021)

Detailed Description

Statement

Reference

The expression of Fs^GD15843 under the control of Scer\GAL4^NP1624 during adulthood (under the additional control of Gal80{ts]) induces a progressive decrease in the number, or even absence, of hub cells in the testes; in those testes where hub cells are absent, both stem cell populations are lost, and only differentiated spermatids can be found.

(Herrera et al., 2021)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development