Allele Dmel\Frq2^{Scer\UAS.cRPa}

General Information
Symbol	Dmel\Frq2^{Scer\UAS.cRPa}	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Romero-Pozuelo	FlyBase ID	FBal0217379
Feature type	allele	Associated gene	Dmel\Frq2

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Romero-Pozuelo et al., 2007)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of a 750bp fragment containing the Frq2 open reading frame. (Romero-Pozuelo et al., 2007)
Carried in construct	P{UAS-Frq2.RP} (Romero-Pozuelo et al., 2007, Dason et al., 2009)
Cytology
Phenotypic Data
Phenotypic Class
	neuroanatomy defective, with Scer\GAL4^D42 (Dason et al., 2009) neuroanatomy defective \| larval stage, with Scer\GAL4^D42 (Romero-Pozuelo et al., 2007) neurophysiology defective, with Scer\GAL4^D42 (Dason et al., 2009) neurophysiology defective \| larval stage, with Scer\GAL4^D42 (Romero-Pozuelo et al., 2007) neurophysiology defective \| larval stage, with Scer\GAL4^elav-C155 (Romero-Pozuelo et al., 2007)
Phenotype Manifest In
	embryonic/larval neuromuscular junction, with Scer\GAL4^D42 (Romero-Pozuelo et al., 2007) embryonic/larval neuromuscular junction, with Scer\GAL4^elav-C155 (Romero-Pozuelo et al., 2007) NMJ bouton, with Scer\GAL4^D42 (Dason et al., 2009) type I bouton, with Scer\GAL4^D42 (Romero-Pozuelo et al., 2007) type I bouton, with Scer\GAL4^elav-C155 (Romero-Pozuelo et al., 2007)
Detailed Description
	Statement Reference Overexpression of Frq2[Scer\UAS.cRPa] under the control of Scer\GAL4[D42] depresses short-term synaptic plasticity in a high Ca[2+] concentration, compared to controls. Expression of Frq2[Scer\UAS.cRPa] does not change the number of active zones per synapse in 1b boutons nor the number of docked vesicles. These mutants do not show any significant difference in resting Ca[2+] level compared to controls. Overexpression of Frq1[Scer\UAS.cRPa] under the control of Scer\GAL4[D42] significantly reduces the number of synaptic boutons. Expression of Frq2[Scer\UAS.cRPa] under the control of Scer\GAL4[D42] at 25[o]C results in reduced nerve-terminal morphology. (Dason et al., 2009) The number of type 1s boutons at the larval neuromuscular junction is reduced compared to wild type in animals expressing Frq2^{Scer\UAS.cRPa} under the control of Scer\GAL4^D42, while the number of type 1b boutons is not significantly affected. The amplitude of the evoked excitatory junctional potential (EJP) at the neuromuscular junction is increased compared to wild type in larvae expressing Frq2^{Scer\UAS.cRPa} under the control of Scer\GAL4^elav-C155 or Scer\GAL4^D42. The amplitude and frequency of spontaneous mEJPs is not affected in these flies. Quantal content of type 1b and type 1s boutons is increased in these flies compared to wild type. (Romero-Pozuelo et al., 2007)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Statement Reference Frq2^{Scer\UAS.cRPa}, Scer\GAL4^D42 has neuroanatomy defective phenotype, non-enhanceable by cac[+]/cac^TS2, Scer\GAL4^D42 (Dason et al., 2009)
Suppressed by
Statement Reference Frq2^{Scer\UAS.cRPa}, Scer\GAL4^D42 has neurophysiology defective phenotype, suppressible by cac[+]/cac^TS2, Scer\GAL4^D42 (Dason et al., 2009)
NOT suppressed by
Statement Reference Frq2^{Scer\UAS.cRPa}, Scer\GAL4^D42 has neuroanatomy defective phenotype, non-suppressible by cac[+]/cac^TS2, Scer\GAL4^D42 (Dason et al., 2009)
NOT Suppressor of
Statement Reference Scer\GAL4^D42/Frq2^{Scer\UAS.cRPa} is a non-suppressor of neuroanatomy defective phenotype of cac^TS2 (Dason et al., 2009) Scer\GAL4^D42/Frq2^{Scer\UAS.cRPa} is a non-suppressor of neurophysiology defective \| temperature conditional phenotype of cac^TS2 (Dason et al., 2009)
Phenotype Manifest In
Suppressed by
Statement Reference Frq2^{Scer\UAS.cRPa}, Scer\GAL4^D42 has NMJ bouton phenotype, suppressible by cac^TS2, Scer\GAL4^D42 (Dason et al., 2009)
NOT Suppressor of
Statement Reference Scer\GAL4^D42/Frq2^{Scer\UAS.cRPa} is a non-suppressor of NMJ bouton phenotype of cac^TS2 (Dason et al., 2009)
Additional Comments
Genetic Interactions
Statement Reference Expression of Frq2[Scer\UAS.cRPa] under the control of Scer\GAL4[D42] in a cac[TS2] background at 25[o]C results in reduced nerve-terminal morphology. Expression of Frq2[Scer\UAS.cRPa] under the control of Scer\GAL4[D42] in a cac[TS2] background at 22[o]C does no change the number of synaptic boutons compared to controls. The presence of a cac[TS2] mutant background suppresses the enhanced neurotransmitter release found upon overexpression of Frq2[Scer\UAS.cRPa] under the control of Scer\GAL4[D42] at both permissive and non-permissive temperatures. (Dason et al., 2009)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	Scer\GAL4^D42/Frq2^{Scer\UAS.cRPa} rescues Df(1)frq^del1 (Dason et al., 2009) Scer\GAL4^D42/Frq2^{Scer\UAS.cRPa} rescues Df(1)frq^del1/Df(1)frq^del3 (Dason et al., 2009) Scer\GAL4^D42/Frq2^{Scer\UAS.cRPa} rescues Df(1)frq^del3 (Dason et al., 2009)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Frq2^{Scer\UAS.cRPa}
Name Synonym	Saccharomyces cerevisiae UAS construct a of Romero-Pozuelo
Secondary FlyBase IDs

References ( 2 )
Research paper	Dason et al., 2009, J. Cell Sci. 122(22): 4109--4121 Frequenin/NCS-1 and the Ca2+-channel α1-subunit co-regulate synaptic transmission and nerve-terminal growth. [FBrf0209325] Romero-Pozuelo et al., 2007, Europ. J. Neurosci. 26(9): 2428--2443 Chronic and acute alterations in the functional levels of Frequenins 1 and 2 reveal their roles in synaptic transmission and axon terminal morphology. [FBrf0200597]

Allele Dmel\Frq2Scer\UAS.cRPa

Allele Dmel\Frq2^{Scer\UAS.cRPa}