FlyBase Allele Report: Dmel\ple[KK101660]

General Information

Symbol

Dmel\ple^KK101660

Species

D. melanogaster

Name

FlyBase ID

FBal0231411

Feature type

allele

Associated gene

Dmel\ple

Associated Insertion(s)

Carried in Construct

P{KK101660}

Also Known As

dsDTH

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK101660}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK101660

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal flight, with Scer\GAL4^Ddc.PU

(Pathak et al., 2015)

abnormal flight, with Scer\GAL4^ple.PF

(Pathak et al., 2015)

abnormal flight, with Scer\GAL4^ple.TH-D

(Pathak et al., 2015)

abnormal neurophysiology | adult stage, with Scer\GAL4^ple.PF

(Sadaf et al., 2015)

flightless, with Scer\GAL4^GMR67E06

(Sadaf et al., 2015)

flightless, with Scer\GAL4^ple.PF

(Sadaf et al., 2015)

uncoordinated | adult stage, with Scer\GAL4^GMR67E06

(Sadaf et al., 2015)

uncoordinated | adult stage, with Scer\GAL4^ple.PF

(Sadaf et al., 2015)

Phenotype Manifest In

dorsal longitudinal indirect flight muscle cell, with Scer\GAL4^ple.PF

(Sadaf et al., 2015)

wing, with Scer\GAL4^GMR67E06

(Sadaf et al., 2015)

wing, with Scer\GAL4^ple.PF

(Sadaf et al., 2015)

Detailed Description

Statement

Reference

Expression of ple^KK101660 under the control of Scer\GAL4^Abd-B.D1 has no apparent effect on the morphology of the secondary cells in male accessory glands.

(Sitnik et al., 2016)

Expression of ple^KK101660 under the control of any one of Scer\GAL4^Ddc.PU, Scer\GAL4^ple.PF, and Scer\GAL4^ple.TH-D leads to significant flight defects, albeit to varying levels.

(Pathak et al., 2015)

Expression of ple^KK101660 under the control of Scer\GAL4^GMR67E06 or Scer\GAL4^ple.PF, in combination with a Gal80[ts] transgene to restrict expression to the adult stage, significantly reduces the number of animals capable of flight, compared to controls; animals that do not fly have significant wing coordination defects, compared to controls; in the case of Scer\GAL4^ple.PF there is also a significant reduction in the duration of electrophysiological spiking in dorsal longitudinal indirect flight muscles in response to an air puff, compared to controls.

(Sadaf et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

ple^KK101660/Scer\GAL4^pnr-MD237 is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4^pnr-MD237, Txl^NIG.5495R

(Uechi et al., 2018)

Other

Statement

Reference

Scer\GAL4^pnr-MD237, Txl^NIG.5495R, ple^KK101660 has visible | adult stage phenotype

(Uechi et al., 2018)

Phenotype Manifest In

Suppressor of

Statement

Reference

ple^KK101660/Scer\GAL4^pnr-MD237 is a suppressor | partially of adult thorax phenotype of Scer\GAL4^pnr-MD237, Txl^NIG.5495R

(Uechi et al., 2018)

Other

Statement

Reference

Scer\GAL4^pnr-MD237, Txl^NIG.5495R, ple^KK101660 has adult thorax phenotype

(Uechi et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

The abnormal dark pigmentation in the central thorax of adult flies expressing Txl^NIG.5495R under the control of Scer\GAL4^pnr-MD237 is strongly suppressed by co-expression of ple^KK101660 (two dark narrow stripes remain at the edges of the expression domain, whereas the rest of it is slightly depigmented and of lighter color than the lateral, wild-type, parts of the thorax).

(Uechi et al., 2018)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v108879

P{KK101660}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

ple^KK101660

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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