Expression of CrebB^KK108927 under the control of Scer\GAL4^VT016811 or Scer\GAL4^VT041043, in combination with a Gal80[ts] transgene to restrict expression to the 5-7 days of the adult stage immediately before, and the 24 h during, experimentation, does not significantly affect aversive olfactory long term memory in adults, compared to controls, when flies undergo spaced training.

1 day memory is impaired after spaced training, but not after massed training in an olfactory associative conditioning assay in flies expressing CrebB-17A^KK108927 under the control of Scer\GAL4^G0431. The defect in memory after spaced training is still seen if the temperature sensitive Scer\GAL80^ts.αTub84B allele is used to restrict Scer\GAL4 expression to the adult stage.