Expression of AP-1-2β^JF02963 under the control of Scer\GAL4^ey.PU induces a headless phenotype in pupae. Expression under the control of Scer\GAL4^elav-C155 or Scer\GAL4^Toll-6-D42 results in active larvae that die as early pupae. Expression under the control of Scer\GAL4^elav-C155 (in combination with Dicer-2 for efficient RNAi) also leads to a significant increase in the number of boutons at the larval muscle 6/7 NMJs, as compared to controls.

Under starvation conditions, the expression of AP-1-2β^JF02963 under the control of Scer\GAL4^Cg.PA results in the third instar larval fat body showing an the accumulation and enlargement of early and late endosomes (identified as GFP-FYVE and GFP-Rab7 cytoplasmic puncta, respectively), an accumulation of lysosomes at the cell membrane (identified as LAMP-GFP) , and an accumulation and enlargement of autophagosomes (identified as GFP-Atg8 cytoplasmic puncta and p62 protein cytoplasmic puncta), as compared to starved controls. However, similar defects in early and late endosomes, lysosomes and autophagosomes are also observed under normal diet.

Expression of AP-1-2β^JF02963 under the control of Scer\GAL4^{SPARC-MI00329-GAL4} results in melanisation of the fat body.