FB2025_01 , released February 20, 2025
Allele: Dmel\daoUAS.cFa
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General Information
Symbol
Dmel\daoUAS.cFa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Fergestad
FlyBase ID
FBal0246073
Feature type
allele
Associated gene
Dmel\dao
Associated Insertion(s)
Carried in Construct
P{UAS-dao.F}
Key Links
Transgenic product class
wild_type
(Fergestad et al., 2010)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Fergestad et al., 2010)
Mutagen
in vitro construct
(Fergestad et al., 2010)
Progenitor genotype
Carried in construct
P{UAS-dao.F}
Cytology
Description

UAS regulatory sequences drive expression of dao.

(Fergestad et al., 2010)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
dao
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of daoScer\UAS.cFa under the control of either Scer\GAL4tub.PU or Scer\GAL4elav-C155 in a wild-type background results in a temperature sensitive paralytic phenotype with rapid kinetics of paralysis and recovery.

      Expression of daoScer\UAS.cFa under the control of Scer\GAL4Mhc.PW does not result in a paralytic phenotype.

      (Fergestad et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      eag1 Sh14 flies show ether-sensitive leg-shaking behavior, which is not suppressed by expression of daoScer\UAS.cFa under the control of Scer\GAL4unspecified.

      (Fergestad et al., 2010)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      daoUAS.cFa/Scer\GAL4elav.PLu rescues daounspecified

      (Fergestad et al., 2010)
      Partially rescues

      daoUAS.cFa partially rescues daounspecified

      (Fergestad et al., 2010)
      Comments

      daoScer\UAS.cFa partially rescues the temperature sensitive paralytic phenotype of daounspecified mutants, even in the absence of a Scer\GAL4 driver. More complete rescue is seen if daoScer\UAS.cFa expression is driven by Scer\GAL4elav.PLu.

      (Fergestad et al., 2010)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      daoScer\UAS.cFa
      daoUAS.cFa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Fergestad
      Secondary FlyBase IDs
        References (1)