Allele Dmel\cmpy^Δ8

General Information
Symbol	Dmel\cmpy^Δ8	Species	D. melanogaster
Name		FlyBase ID	FBal0261239
Feature type	allele	Associated gene	Dmel\cmpy

Allele class	amorphic allele - molecular evidence, amorphic allele - genetic evidence
Mutagen	FLPase
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	amorphic allele - genetic evidence (James and Broihier, 2011) amorphic allele - molecular evidence (James and Broihier, 2011)
Mutagen	FLPase (James and Broihier, 2011)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	PBac{WH}cmpy^f02482 (James and Broihier, 2011) PBac{WH}f01736 (James and Broihier, 2011)
Nature of the lesion	Statement Reference Deletion of approximately 3 kb of upstream sequence, the translation initiation site and the majority of the coding sequence of cmpy. (James and Broihier, 2011)
Caused by insertion	PBac{WHr}cmpy^Δ8 (James and Broihier, 2011)
Caused by aberration	Df(3L)cmpy^Δ8 (James and Broihier, 2011)
Cytology
Phenotypic Data
Phenotypic Class
	female sterile \| recessive (James and Broihier, 2011) neuroanatomy defective \| recessive \| third instar larval stage (James and Broihier, 2011) viable (James and Broihier, 2011)
Phenotype Manifest In
	type I bouton \| supernumerary \| third instar larval stage (James and Broihier, 2011) type I bouton \| supernumerary \| third instar larval stage (with Df(3L)BSC452) (James and Broihier, 2011) type I bouton \| third instar larval stage (James and Broihier, 2011)
Detailed Description
	Statement Reference cmpy[Δ8] homozygous mutants are viable, although females are sterile. cmpy[Δ8] homozygotes do not exhibit defects in neuronal cell fate acquisition or axon guidance. cmpy[Δ8] homozygotes display a 52% increase in the number of boutons at neuromuscular junction (NMJ) 6/7 and a 57% increase in type I boutons at NMJ 4. cmpy[Δ8]/Df(3L)BSC452 larvae show a comparable phenotype. cmpy[Δ8] mutant boutons tend to be smaller than those of wild type. cmpy[Δ8] mutants display a 2.9-fold increase in the number of satellite boutons at NMJ 4. cmpy[Δ8] mutant NMJs contain more active zones, but the percentage increase is comparable to that in bouton number, such that the active zone density remain constant. The assembly of periactive zones appears normal. (James and Broihier, 2011)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
Statement Reference cmpy^Δ8 has type I bouton \| supernumerary \| third instar larval stage phenotype, suppressible \| partially by gbb¹/gbb[+] (James and Broihier, 2011) cmpy^Δ8 has type I bouton \| supernumerary \| third instar larval stage phenotype, suppressible \| partially by Mad[+]/Mad¹ (James and Broihier, 2011) cmpy^Δ8 has type I bouton \| supernumerary \| third instar larval stage phenotype, suppressible \| partially by wit^A12/wit[+] (James and Broihier, 2011) cmpy^Δ8 has type I bouton \| supernumerary \| third instar larval stage phenotype, suppressible by gbb^{dsRNA.Scer\UAS.cBa}/Scer\GAL4^D42 (James and Broihier, 2011)
Other
Statement Reference cmpy^Δ8, wit^A12 has type I bouton \| third instar larval stage phenotype (James and Broihier, 2011)
Additional Comments
Genetic Interactions
Statement Reference The neuromuscular junction expansion phenotype seen in cmpy[Δ8] mutants is partially suppressed when animals are also heterozygous for gbb[1], Mad[1] or wit[A12]. wit[A12], cmpy[Δ8] double mutants show a 44% reduction in bouton number at neuromuscular junction 4, thereby phenocopying the neuromuscular junction undergrowth displayed by wit single mutants. Scer\GAL4[D42]-mediated expression of gbb[dsRNA.Scer\UAS.cBa] suppresses the neuromuscular junction expansion phenotype seen in cmpy[Δ8] mutants. (James and Broihier, 2011)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Partially rescued by	cmpy^Δ8 is partially rescued by cmpy^Scer\UAS.cJa/Scer\GAL4^D42 (James and Broihier, 2011) cmpy^Δ8 is partially rescued by cmpy^Scer\UAS.cJa/Scer\GAL4^elav.PU (James and Broihier, 2011)
Comments	Scer\GAL4[D42]-mediated overexpression of cmpy[Scer\UAS.cJa] in a cmpy[Δ8] background fully rescues bouton number at neuromuscular junction (NMJ) 4 whereas overgrowth is reduced from 52% to 9% at NMJ 6/7. Comparable rescue is observed when Scer\GAL4[elav.PU] is used. (James and Broihier, 2011)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	CG13253Δ8 (James and Broihier, 2011) cmpy^Δ8 (Nechipurenko and Broihier, 2012, James and Broihier, 2011)
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Nechipurenko and Broihier, 2012, J. Cell Biol. 196(3): 345--362 FoxO limits microtubule stability and is itself negatively regulated by microtubule disruption. [FBrf0217391] James and Broihier, 2011, Development 138(15): 3273--3286 Crimpy inhibits the BMP homolog Gbb in motoneurons to enable proper growth control at the Drosophila neuromuscular junction. [FBrf0214390]

Allele Dmel\cmpyΔ8

Allele Dmel\cmpy^Δ8