FlyBase Allele Report: Dmel\Caf1-55[p55-1]

General Information

Symbol

Dmel\Caf1-55^p55-1

Species

D. melanogaster

Name

FlyBase ID

FBal0267805

Feature type

allele

Associated gene

Dmel\Caf1-55

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Wen et al., 2012)

Mutagen

Delta2-3 transposase

(Wen et al., 2012)

Progenitor genotype

P{SUPor-P}CG31344^KG02618

(Wen et al., 2012)

Cytology

Description

Imprecise excision of the P{SUPor-P} element in P{SUPor-P}CG31344^KG02618 resulted in a 2244bp deletion which removes the entire Caf1 locus and 9bp of 3'UTR of neighbouring gene Art3.

(Wen et al., 2012)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3R:15,225,357..15,227,600 [+]

Comment:

Inferred boundaries of a 2244 bp deletion resulting from the imprecise excision of P{SUPor-P}CG31344^KG02618, which removes the Caf1-55 coding region.

(Wen et al., 2012)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Homozygous Caf1^p55-1 mutants are lethal before pupariation. Larvae are 1-2 days developmentally delayed compared to wild type and the body size is greatly reduced. Some mutant larvae survive for up to 6-8 days, but they never undergo pupariation.

Caf1^p55-1/Caf1^p55-2 mutants are 1-2 days developmentally delayed compared to wild type and the body size is greatly reduced. Some mutant larvae survive for up to 6-8 days, but larvae never undergo pupariation.

Homozygous mutant Caf1^p55-1 germline stem cell clones in females are lost more quickly than in controls. TUNEL staining shows that this is due to differentiation rather than apoptosis.An increased number of homozygous mutant Caf1^p55-1 follicle clones undergo cell death compared to controls.

Caf1^p55-1 mutant clones in larval wing and eye discs are significantly smaller than wild type controls.

One copy of Caf1^p55-1 is unable to suppress the variegation seen in In(1)w^m4h/+.

(Wen et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Caf1-55^p55-1 has lethal | second instar larval stage phenotype, non-suppressible by CG31344^tWa

(Wen et al., 2012)

Caf1-55^p55-1 has lethal | second instar larval stage phenotype, non-suppressible by Scer\GAL4^Act5C.PI/Hsap\RBBP4^UAS.cWa

(Wen et al., 2012)

Caf1-55^p55-1 has lethal | second instar larval stage phenotype, non-suppressible by Hsap\RBBP7^UAS.cWa/Scer\GAL4^Act5C.PI

(Wen et al., 2012)

Phenotype Manifest In

Suppressed by

Statement

Reference

Caf1-55^p55-1 has follicle cell | somatic clone | adult stage phenotype, suppressible | partially by Scer\GAL4^Act5C.PI/Hsap\RBBP4^UAS.cWa

(Wen et al., 2012)

Caf1-55^p55-1 has follicle cell | somatic clone | adult stage phenotype, suppressible | partially by Hsap\RBBP7^UAS.cWa/Scer\GAL4^Act5C.PI

(Wen et al., 2012)

NOT Enhancer of

Statement

Reference

Caf1[+]/Caf1-55^p55-1 is a non-enhancer of sex comb | ectopic phenotype of Pc¹⁵

(Wen et al., 2012)

NOT Suppressor of

Statement

Reference

Caf1[+]/Caf1-55^p55-1 is a non-suppressor of sex comb | ectopic phenotype of Pc¹⁵

(Wen et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

CG31344^tWa is unable to rescue the lethality seen in homozygous Caf1^p55-1 mutants.

Caf1^p55-1/+ does not affect the ectopic sex comb phenotype seen in Pc¹⁵ heterozygotes.

(Wen et al., 2012)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\RBBP7^Scer\UAS.cWa under the control of Scer\GAL4^Act5C.PI partially rescues the viability and proliferation defects seen in Caf1^p55-1 mutant follicle cell clones.

Expression of Hsap\RBBP4^Scer\UAS.cWa under the control of Scer\GAL4^Act5C.PI partially rescues the viability and proliferation defects seen in Caf1^p55-1 mutant follicle cell clones.

Expression of Hsap\RBBP4^Scer\UAS.cWa under the control of Scer\GAL4^Act5C.PI does not rescue the lethality seen in Caf1^p55-1 homozygous mutants.

Expression of Hsap\RBBP7^Scer\UAS.cWa under the control of Scer\GAL4^Act5C.PI does not rescue the lethality seen in Caf1^p55-1 homozygous mutants.

(Wen et al., 2012)

Complementation and Rescue Data

Rescued by

Caf1-55^p55-1 is rescued by Caf1-55^+t1.3p55

(Wen et al., 2012)

Caf1-55^p55-1 is rescued by Scer\GAL4^Act5C.PI/Caf1-55^UAS.cWa

(Wen et al., 2012)

Caf1-55^p55-2/Caf1-55^p55-1 is rescued by Caf1-55^+t1.3p55

(Wen et al., 2012)

Partially rescued by

Caf1-55^p55-1 is partially rescued by Caf1-55^1.3p55m

(Wen et al., 2012)

Caf1-55^p55-2/Caf1-55^p55-1 is partially rescued by Caf1-55^1.3p55m

(Wen et al., 2012)

Not rescued by

Caf1-55^p55-2/Caf1-55^p55-1 is not rescued by Caf1-55^t0.5p55

(Wen et al., 2012)

Comments

Expression of Caf1^+t1.3p55 fully rescues the lethality and developmental delay phenotypes seen in Caf1^p55-1/Caf1^p55-2 mutants.

Expression of Caf1^t0.5p55 is unable to rescue the lethality and developmental delay phenotypes seen in Caf1^p55-1/Caf1^p55-2 mutants.

Expression of Caf1^Scer\UAS.cWa under the control of Scer\GAL4^Act5C.PI rescues the cell death seen phenotype seen in Caf1^p55-1 mutant follicle cell clones.

Expression of Caf1^+t1.3p55 fully rescues the germline stem cell loss and follicle cell proliferation phenotypes seen in Caf1^p55-1 mutant clones.

Expression of Caf1^1.3p55m partially rescues the lethality and developmental delay phenotypes seen in Caf1^p55-1/Caf1^p55-2 mutants. The rescued flies are fertile and can be maintained as a stable stock.

Expression of Caf1^1.3p55m partially rescues the germline stem cell loss and follicle cell proliferation phenotypes seen in Caf1^p55-1 mutant clones.

(Wen et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

68168

w^*; Caf1-55^p55-1/TM3, Sb¹

79046

w^*; P{neoFRT}82B Caf1-55^p55-1/TM3, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Caf1-55^p55-1

(Li et al., 2023)

Caf1^p55-1

p55¹

(Wen et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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