FB2025_01 , released February 20, 2025
Allele: Dmel\TBPHUAS.cHa
Open Close
General Information
Symbol
Dmel\TBPHUAS.cHa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Hazelett
FlyBase ID
FBal0277270
Feature type
allele
Associated gene
Dmel\TBPH
Associated Insertion(s)
Carried in Construct
P{UAS-TBPH.H}
Also Known As
UAS-TBPH
Key Links
Transgenic product class
cDNA
(Hazelett et al., 2012)
wild_type
(Hazelett et al., 2012)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Hazelett et al., 2012)
wild_type
(Hazelett et al., 2012)
Mutagen
in vitro construct
(Hazelett et al., 2012)
Progenitor genotype
Carried in construct
P{UAS-TBPH.H}
Cytology
Description

UASt regulatory sequences drive expression of a 1.8kb TBPH cDNA.

(Hazelett et al., 2012)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
TBPH
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  amyotrophic lateral sclerosis type 10
      CEA
      (Pons et al., 2017)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Larvae expressing TBPHScer\UAS.cHa under the control of Scer\GAL4D42 show normal locomotor behavior. However, adults show significant climbing defects within a week of eclosion.

      (Hazelett et al., 2012)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      Scer\GAL4TBPH.PH/TBPHUAS.cHa partially rescues TBPHG2

      (Lembke et al., 2017)

      Scer\GAL4TBPH.PH/TBPHUAS.cHa partially rescues TBPHG2

      (Chang et al., 2014)

      Scer\GAL4Toll-6-D42/TBPHUAS.cHa partially rescues TBPHG2

      (Hazelett et al., 2012)

      Scer\GAL4TBPH.PH/TBPHUAS.cHa partially rescues TBPHG2

      (Hazelett et al., 2012)
      Fails to rescue

      Scer\GAL4GMR75C05/TBPHUAS.cHa fails to rescue TBPHG2

      (Lembke et al., 2017)
      Comments

      The expression of TBPHScer\UAS.cHa under the control of Scer\GAL4TBPH.PH, but not of Scer\GAL4GMR75C05, partially rescues the decreased locomotor capacity in crawling assays, as well as the associated slower and less frequent peristaltic waves, observed in TBPHG2 third instar larvae.

      The expression of TBPHScer\UAS.cHa under the control of Scer\GAL4TBPH.PH also partially suppresses the decreased amplitude, but not the decreased frequency, of spontaneous miniature endplate potentials in the neurotransmission across the TBPHG2 larval neuromuscular junction. This expression also rescues the decrease in motor bursts and the increased delay between A7 and A2 motor bursts observed TBPHG2 mutant larvae.

      (Lembke et al., 2017)

      Expression of TBPHScer\UAS.cHa driven by Scer\GAL4TBPH.PH significantly partially rescues locomotor defects in TBPHG2/TBPHG2 third instar larvae.

      (Chang et al., 2014)

      Expression of TBPHScer\UAS.cHa under the control of Scer\GAL4D42 partially rescues the locomotor defects of TBPHG2 larvae.

      Expression of TBPHScer\UAS.cHa under the control of either Scer\GAL4D42 or Scer\GAL4TBPH.PH partially rescues the lethality of TBPHG2 animals.

      (Hazelett et al., 2012)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      TBPHScer\UAS.cHa
      TBPHUAS.cHa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Hazelett
      Secondary FlyBase IDs
        References (4)