FB2025_01 , released February 20, 2025
Allele: Dmel\AatfUASp.EGFP
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General Information
Symbol
Dmel\AatfUASp.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0287852
Feature type
allele
Associated gene
Dmel\Aatf
Associated Insertion(s)
Carried in Construct
P{UASp-Aatf.EGFP}
Key Links
Transgenic product class
cDNA
(Jagut et al., 2013)
wild_type
(Jagut et al., 2013)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Jagut et al., 2013)
wild_type
(Jagut et al., 2013)
Mutagen
in vitro construct
(Jagut et al., 2013)
Progenitor genotype
Carried in construct
P{UASp-Aatf.EGFP}
Cytology
Description

UASp regulatory sequences drive expression of a full-length Aatf cDNA (clone RE30678) tagged at the C-terminal end with EGFP.

(Jagut et al., 2013)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
Aatf
Tagged with
EGFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Flies expressing AatfScer\UAS.P\T.T:Avic\GFP-EGFP under the control of Scer\GAL4os.cXa have an increased number of polar cells per egg chamber compared to wild type; all wild type egg chambers have two polar cells per cluster, whereas 6% of polar cell clusters contain more than two cells.

      (Jagut et al., 2013)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4VP16.nanos.UTR/AatfUASp.EGFP rescues Aatfnutmeg-21.3

      (Jagut et al., 2013)

      Scer\GAL4VP16.nanos.UTR/AatfUASp.EGFP rescues Aatfnutmeg-29.3

      (Jagut et al., 2013)
      Comments

      Expression of AatfScer\UAS.P\T.T:Avic\GFP-EGFP under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 fully rescues the egg chamber arrest and oocyte polarity defects seen in Aatfnutmeg-21.3 mutant germline clones.

      Expression of AatfScer\UAS.P\T.T:Avic\GFP-EGFP under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 fully rescues the egg chamber arrest and oocyte polarity defects seen in Aatfnutmeg-29.3 mutant germline clones.

      (Jagut et al., 2013)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      AatfScer\UAS.P\T.T:Avic\GFP-EGFP
      AatfUASp.EGFP
      Name Synonyms
      Secondary FlyBase IDs
        References (1)