FB2025_01 , released February 20, 2025
Allele: Dmel\Usp519-2
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General Information
Symbol
Dmel\Usp519-2
Species
D. melanogaster
Name
FlyBase ID
FBal0298995
Feature type
allele
Associated gene
Dmel\Usp5
Associated Insertion(s)
Carried in Construct
Also Known As
19-2
Key Links
Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Wang et al., 2014)
Mutagen
P-element activity
(Wang et al., 2014)
Progenitor genotype
P{EPgy2}BtbVIIEY07802
(Wang et al., 2014)
Caused by aberration
Df(3L)Usp519-2
Cytology
Description

Imprecise excision of P{EPgy2}BtbVIIEY07802, resulting in deletion of a 1kb region including the shared promoter region of BtbVII and Usp5 and the predicted ATG codon of Usp5.

(Wang et al., 2014)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Usp5leon-1/Usp519-2 as well as Usp5leon-2/Usp519-2 mutant third instar larvae display neuromuscular junction (NMJ) defects and the synaptic boutons are aggregated and less numerous relative to wild-type controls, and in Usp5leon-2/Usp519-2 the total branch length is also significantly decreased. Muscle area is not different from controls.

      Although the overall neuromuscular junction bouton area in either Usp519-2 homozygous or Usp5leon-1/Usp519-2 transheterozygous mutant larvae is comparable to controls, the area of the subsynaptic reticulum surrounding it is much larger and the average length of synaptic membranes is also increased, whereas the number of T-bars per bouton or the vesicle density is not significantly different from controls. The electrophysiological properties of the Usp519-2 and Usp5leon-1/Usp519-2 mutant NMJs are also perturbed: while both the frequency and amplitude of miniature excitatory junction current (mEJC) is not significantly different compared to Usp519-2 heterozygous controls, the EJC amplitude and quantal content is significantly reduced.

      (Wang et al., 2017)

      Usp5leon-1/Usp519-2 larvae appear normal in body size, but brain lobes and wing disks are smaller than normal. Larval growth is also slower compared to controls.

      (Wang et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Usp519-2 is rescued by Usp5GFP

      (Wang et al., 2017)

      Usp519-2/Usp5leon-1 is rescued by Usp5GFP

      (Wang et al., 2017)

      Usp519-2/Usp5leon-1 is rescued by Scer\GAL4how-24B/Usp5UAS.Tag:FLAG

      (Wang et al., 2017)

      Usp519-2 is rescued by Usp5GFP

      (Wang et al., 2014)

      Usp519-2/Usp5leon-1 is rescued by Scer\GAL4Tub.PU/Usp5UAS.Tag:FLAG

      (Wang et al., 2014)
      Not rescued by

      Usp519-2/Usp5leon-1 is not rescued by Usp5UAS.Tag:FLAG/Scer\GAL4Toll-6-D42

      (Wang et al., 2017)

      Usp519-2/Usp5leon-1 is not rescued by Scer\GAL4how-24B/Usp5ED.UAS.Tag:FLAG

      (Wang et al., 2017)

      Usp519-2/Usp5leon-1 is not rescued by Scer\GAL4Toll-6-D42/Usp5ED.UAS.Tag:FLAG

      (Wang et al., 2017)

      Usp519-2/Usp5leon-1 is not rescued by Scer\GAL4Tub.PU/Usp5ED.UAS.Tag:FLAG

      (Wang et al., 2014)
      Comments

      The neuromuscular junction morphological defects (decreased total branch length, reduced number of boutons) observed in Usp5leon-1/Usp519-2 mutant third instar larvae are fully rescued by combination with Usp5T:Avic\GFP or by expression of Usp5Scer\UAS.T:Zzzz\FLAG driven by Scer\GAL4how-24B but not if driven by Scer\GAL4Toll-6-D42, expression of Usp5ED.Scer\UAS.T:Zzzz\FLAG fails to rescue the morphology regardless of the driver.

      Usp5T:Avic\GFP and Usp5Scer\UAS.T:Zzzz\FLAG expression (with the Scer\GAL4how-24B driver and not with Scer\GAL4Toll-6-D42) also rescues the impaired electrophysiological properties at Usp519-2 or Usp5leon-1/Usp519-2 mutant neuromuscular junctions (reduced excitatory junction current amplitude and quantal content).

      (Wang et al., 2017)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      19-2
      (Wang et al., 2017, Wang et al., 2014)
      Usp519-2
      Name Synonyms
      Secondary FlyBase IDs
        References (2)