FB2025_01 , released February 20, 2025
Allele: Dmel\tut3
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General Information
Symbol
Dmel\tut3
Species
D. melanogaster
Name
FlyBase ID
FBal0302044
Feature type
allele
Associated gene
Dmel\tut
Associated Insertion(s)
TI{TI}tut3
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Chen et al., 2014)
Mutagen
gene targeting by homologous recombination
(Chen et al., 2014)
Progenitor genotype
Associated Insertion(s)
TI{TI}tut3
Cytology
Description

The genomic coding region of tut is replaced with an attP site and a loxP site.

(Chen et al., 2014)
Allele components
Component
Use(s)
Also carries
attP
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
delins
3L:8,209,858..8,210,671 [-]
Comment:

Using 3.1 kb upstream from the start codon and 3.1 kb downstream from the stop codon for homologous recombination, the tut coding region was deleted and replaced with 'attP' and 'loxP'.

(Chen et al., 2014)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Mutants exhibit germ cell overgrowth - male germ cells arrest at the spermatogonial transit-amplifying stage and over-proliferate. The testis lacks later germ cells such as spermatocytes.

      (Chen et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressor of
      Statement
      Reference

      tut1/tut3 is a suppressor of male germline stem cell phenotype of Scer\GAL4nanos.PU, bamUASp.GFP

      (Chen et al., 2014)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Scer\GAL4nos.PU-mediated expression of bamScer\UAS.P\T.T:Avic\GFP in a tut1/tut3 background results in a tut mutant phenotype.

      (Chen et al., 2014)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      tut1/tut3 is rescued by tutUAS.GFP/Scer\GAL4VP16.bam

      (Chen et al., 2014)

      tut3 is rescued by tuttutP.GFP

      (Chen et al., 2014)
      Not rescued by

      tut1/tut3 is not rescued by tutUAS.GFP/Scer\GAL4tj.PU

      (Chen et al., 2014)
      Comments

      Scer\GAL4bam.T:Hsim\VP16-mediated expression, but not Scer\GAL4tj.PU-mediated expression of tutScer\UAS.T:Avic\GFP rescues the testis phenotype of tut1/tut3.

      tuttutP.T:Avic\GFP rescues the testis phenotype of tut3.

      (Chen et al., 2014)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      tut3
      (Shan et al., 2017, Chen et al., 2014)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)