FB2025_01 , released February 20, 2025
Allele: Hsap\APPAβ42.UAS.Tag:SS(nec).cBa
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General Information
Symbol
Hsap\APPAβ42.UAS.Tag:SS(nec).cBa
Species
H. sapiens
Name
Saccharomyces cerevisiae UAS construct a of Burnouf
FlyBase ID
FBal0314799
Feature type
allele
Associated gene
Hsap\APP
Associated Insertion(s)
Carried in Construct
P{UAS-Aβ42.B}
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Burnouf et al., 2015)
Progenitor genotype
Carried in construct
P{UAS-Aβ42.B}
Cytology
Description

UASt regulatory sequences drive expression of amino acids 1-42 of Hsap\APP bearing a secretion signal from nec (Tag:SS(nec)).

(Burnouf et al., 2015)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Hsap\APP
Tagged with
Tag:SS(nec)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  Alzheimer's disease
      CEA
      (Burnouf et al., 2015)
      model of  Alzheimer's disease 1
      CEC
      (Gorsky et al., 2017)
      CEA
      (Xue et al., 2022)
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      model of  Alzheimer's disease 1
      is ameliorated by Hsap\MAPTKI.hMAPT.4Q
      (Gorsky et al., 2017)
      is exacerbated by Hsap\MAPTKI.hMAPT.4R
      (Gorsky et al., 2017)
      is ameliorated by NagluUAS.Tag:HA
      (Xue et al., 2022)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Hsap\APPAβ42.Scer\UAS.T:SS-nec.cBa under the control of Scer\GAL4GMR.PU results in ommatidial disorganisation and eye roughening.

      Adult-restricted neuronal expression of Hsap\APPAβ42.Scer\UAS.T:SS-nec.cBa under the control of Scer\GAL4elav.Switch.PO results in climbing defects and survival. Progressive loss of photoreceptor neurons is seen.

      Adult-restricted neuronal expression of one copy each of Hsap\APPAβ42.Scer\UAS.T:SS-nec.cBa and Hsap\APPAβ40.Scer\UAS.T:SS-nec.cBa under the control of Scer\GAL4elav.Switch.PO results in climbing defects and shortened lifespan that are not seen when one copy of either transgene is expressed alone.

      Adult-restricted neuronal expression of one copy each of Hsap\APPAβ42.Scer\UAS.T:SS-nec.cBa and Hsap\APPAβ43.Scer\UAS.T:SS-nec.cBa under the control of Scer\GAL4elav.Switch.PO results in climbing defects and shortened lifespan that are modestly but significantly less severe than those seen when Hsap\APPAβ42.Scer\UAS.T:SS-nec.cBa is expressed alone.

      (Burnouf et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Hsap\APPAβ42.Scer\UAS.T:SS-nec.cBa
      Hsap\APPAβ42.UAS.Tag:SS(nec).cBa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Burnouf
      Secondary FlyBase IDs
        References (3)