FlyBase Allele Report: Dmel\Atg1[TRiP.cUa]

General Information

Symbol

Dmel\Atg1^TRiP.cUa

Species

D. melanogaster

Name

FlyBase ID

FBal0319615

Feature type

allele

Associated gene

Dmel\Atg1

Associated Insertion(s)

Carried in Construct

P{TRiP.Atg1.U}

Key Links

Transgenic product class

RNAi_reagent

(David-Morrison et al., 2016)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(David-Morrison et al., 2016)

Mutagen

in vitro construct

(David-Morrison et al., 2016)

Progenitor genotype

Carried in construct

P{TRiP.Atg1.U}

Cytology

Description

UAS regulatory sequences drive expression of a Atg1 RNAi construct.

(David-Morrison et al., 2016)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Atg1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

majority die during embryonic stage | maternal effect, with Scer\GAL4^{VP16.mat.αTub67C}

(Kuhn et al., 2015)

Phenotype Manifest In

autophagosome | embryonic stage | maternal effect, with Scer\GAL4^{VP16.mat.αTub67C}

(Kuhn et al., 2015)

lipid droplet | adult stage, with Scer\GAL4^Mhc.PK

(Yan et al., 2017)

mesothoracic tergotrochanter muscle cell, with Scer\GAL4^Mhc.PK

(Yan et al., 2017)

Detailed Description

Statement

Reference

Adult females expressing Atg1^TRiP.cUa under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} produce embryos that fail to form autophagosomes at cellularization (which itself appears normal). These embryos also display a much reduced hatching rate.

(Kuhn et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Atg1^TRiP.cUa, Scer\GAL4^{VP16.mat.αTub67C} has majority die during embryonic stage | maternal effect phenotype, suppressible | maternal effect | partially by mTor^TRiP.cUa, Scer\GAL4^{VP16.mat.αTub67C}

(Kuhn et al., 2015)

Suppressor of

Statement

Reference

Atg1^TRiP.cUa, Scer\GAL4^{VP16.mat.αTub67C} is a suppressor | maternal effect | partially of majority die during embryonic stage | maternal effect phenotype of Scer\GAL4^{VP16.mat.αTub67C}, mTor^TRiP.cUa

(Kuhn et al., 2015)

Phenotype Manifest In

Suppressor of

Statement

Reference

Atg1^TRiP.cUa/Scer\GAL4^Cg.PU is a suppressor of autophagosome | somatic clone | third instar larval stage phenotype of wcy^A

(David-Morrison et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Atg1^TRiP.cUa driven by Scer\GAL4^cg.PU suppresses increases in autophagosomes seen in wcy^A/wcy^A fat body third instar larval clones.

(David-Morrison et al., 2016)

The low hatching rate of embryos from mothers expressing either Tor^TRiP.cUa or Atg1^TRiP.cUa under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} is nearly completely restored when the two transgenes are expressed simultaneously.

(Kuhn et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Atg1^TRiP.cUa

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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