FB2025_01 , released February 20, 2025
Allele: Dmel\mTorTRiP.cUa
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General Information
Symbol
Dmel\mTorTRiP.cUa
Species
D. melanogaster
Name
FlyBase ID
FBal0340158
Feature type
allele
Associated gene
Dmel\mTor
Associated Insertion(s)
Carried in Construct
P{TRiP.mTor.U}
Key Links
Transgenic product class
RNAi_reagent
(Kuhn et al., 2015)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Kuhn et al., 2015)
Mutagen
in vitro construct
(Kuhn et al., 2015)
Progenitor genotype
Carried in construct
P{TRiP.mTor.U}
Cytology
Description

UAS regulatory sequences drive expression of a Tor RNAi construct.

(Kuhn et al., 2015)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
mTor
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Females expressing TorTRiP.cUa under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 produce embryos that are smaller than wild-type and display a much decreased hatching rate, show DNA fragmentation and clumping following syncytial divisions, defective cellularization and increased cell death. Additionally, swaths of multivesicular compartments are present throughout the disorganized cytoplasm. These embryos also display abnormal yolk morphology (decondensation of the vitellogenin mass) during and post-cellularization (the yolk appears normal prior to cellularization, although some yolk platelets are mis-localized near nuclei).

      Expression of TorTRiP.cUa under the combined control of Scer\GAL4nos.UTR.T:Hsim\VP16, Scer\GAL4nos.PG and Scer\GAL4otu.T:Hsim\VP16 prevents germaria formation in females, only terminal filaments remain.

      (Kuhn et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The low hatching rate of embryos from mothers expressing either TorTRiP.cUa or Atg1TRiP.cUa under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 is nearly completely restored when the two transgenes are expressed simultaneously. No hatching rate defects are observed in embryos from Atg2HMS01198 expressing mothers and no rescue effect is observed when Atg2HMS01198 is co-expressed with TorTRiP.cUa.

      (Kuhn et al., 2015)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      TorTRiP.cUa
      mTorTRiP.cUa
      Name Synonyms
      Secondary FlyBase IDs
        References (1)