FB2025_01 , released February 20, 2025
Allele: Dmel\DCTN2-p50RNAi.A.UAS.V22
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General Information
Symbol
Dmel\DCTN2-p50RNAi.A.UAS.V22
Species
D. melanogaster
Name
FlyBase ID
FBal0323827
Feature type
allele
Associated gene
Dmel\DCTN2-p50
Associated Insertion(s)
Carried in Construct
P{UAS-DCTN2-p50.RNAi.A}
Key Links
Transgenic product class
RNAi_reagent
(Liu et al., 2015)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Liu et al., 2015)
Mutagen
in vitro construct
(Liu et al., 2015)
Progenitor genotype
Carried in construct
P{UAS-DCTN2-p50.RNAi.A}
Cytology
Description

UAS regulatory sequences (derived from the Valium22 vector) drive expression of an shRNA that targets DCTN2-p50.

(Liu et al., 2015)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
DCTN2-p50
Also carries
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of DCTN2-p50dsRNA.A.Scer\UAS.P\T under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 results in yolk depletion (measured by loss of yolk autofluorescence), appearance of abnormal large vesicles, delocalization of endocytic and fate determination factors (osk mRNA) from the oocyte cortex, compromised endocytic uptake and altered microtubule organization in the oocytes. On the ultrastructural level, these oocytes show significantly fewer yolk granules as well as cortical coated pits and vesicles and contain numerous endocytic intermediates (e.g. vesicles with partially condensed yolk) compared to controls.

      (Liu et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The number of oocytes containing abnormal large vesicles observed upon DCTN2-p50dsRNA.A.Scer\UAS.P\T-driven expression of Scer\GAL4mat.αTub67C.T:Hsim\VP16 is significantly reduced by co-expression of Rab5S43N.Scer\UAS.

      (Liu et al., 2015)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescued by

      DCTN2-p50RNAi.A.UAS.V22 is partially rescued by DCTN2-p50vas.GFP/Scer\GAL4VP16.mat.αTub67C

      (Liu et al., 2015)
      Comments

      The yolk depletion and the endocytosis defects as well as the delocalization of osk mRNA observed in egg chambers expressing DCTN2-p50dsRNA.A.Scer\UAS.P\T under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 is significantly improved by co-expression of DCTN2-p50vas.T:Avic\GFP.

      (Liu et al., 2015)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      DCTN2-p50RNAi.A.UAS.V22
      DCTN2-p50dsRNA.A.Scer\UAS.P\T
      DCTN2-p50dsRNA.A.UAS.V22
      Name Synonyms
      Secondary FlyBase IDs
        References (2)