FB2025_01 , released February 20, 2025
Allele: Cele\unc-104::KhcLZ.mutA.UASp.mTagBFP2
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General Information
Symbol
Cele\unc-104::KhcLZ.mutA.UASp.mTagBFP2
Species
C. elegans
Name
FlyBase ID
FBal0326711
Feature type
allele
Associated gene
Cele\unc-104::Khc
Associated Insertion(s)
Carried in Construct
P{UASp-UncLZ.Khc.mutA.mTagBFP2}
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Winding et al., 2016)
Progenitor genotype
Carried in construct
P{UASp-UncLZ.Khc.mutA.mTagBFP2}
Cytology
Description

UASp regulatory sequences drive expression of the Cele\unc-104 motor domain (amino acids 1-389) along with a leucine zipper for dimerization, fused to the mutated C-terminus of Khc (amino acids 905-975; with R914A, K915A, R916A and Q918A mutations in the microtubule binding site). The construct is tagged with mTagBFP2 at the C-terminal.

(Winding et al., 2016)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
Cele\unc-104::Khc
Tagged with
mTagBFP2
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      The reduced velocity of ooplasmic streaming characteristic for oocytes expressing KhcGL00330 under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 cannot be rescued by co-expression of Cele\unc-104::KhcLZ.mutA.Scer\UAS.P\T.T:Equa\eqFP578-TagBFP2.

      (Lu et al., 2016)

      The reduced axon length observed in cultured neurons from KhcKO.mutA mutant larvae as well as the axon outgrowth and patterning of photoreceptor neurons in KhcKO.mutA larval brains cannot be rescued by expression of Cele\unc-104::KhcLZ.mutA.Scer\UAS.P\T.T:Equa\eqFP578-TagBFP2 under the control of Scer\GAL4elav.PLu in the mutant background.

      (Winding et al., 2016)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Cele\unc-104::KhcLZ.mutA.Scer\UAS.P\T.T:Equa\eqFP578-TagBFP2
      Cele\unc-104::KhcLZ.mutA.UASp.TagBFP2
      Cele\unc-104::KhcLZ.mutA.UASp.mTagBFP2
      Name Synonyms
      Secondary FlyBase IDs
        References (2)