FlyBase Allele Report: Cele\unc-104[LZ.UASp.mTagBFP2]

General Information

Symbol

Cele\unc-104^{LZ.UASp.mTagBFP2}

Species

C. elegans

Name

FlyBase ID

FBal0326712

Feature type

allele

Associated gene

Cele\unc-104

Associated Insertion(s)

Carried in Construct

P{UASp-UncLZ.mTagBFP2}

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Winding et al., 2016)

Progenitor genotype

Carried in construct

P{UASp-UncLZ.mTagBFP2}

Cytology

Description

UASp regulatory sequences drive expression of the Cele\unc-104 motor domain (amino acids 1-389) along with a leucine zipper for dimerization, tagged with mTagBFP2 at the C-terminal.

(Winding et al., 2016)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

Cele\unc-104

Tagged with

mTagBFP2

blue fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

Cele\unc-104^{LZ.UASp.mTagBFP2}/Scer\GAL4^elav.PLu is a non-suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Khc^KO.mutA

(Winding et al., 2016)

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

Cele\unc-104^{LZ.UASp.mTagBFP2}, Scer\GAL4^{VP16.nanos.UTR} is a non-suppressor of oocyte phenotype of Khc^GL00330, Scer\GAL4^{VP16.nanos.UTR}

(Lu et al., 2016)

Cele\unc-104^{LZ.UASp.mTagBFP2}/Scer\GAL4^elav.PLu is a non-suppressor of neuron | third instar larval stage phenotype of Khc^KO.mutA

(Winding et al., 2016)

Cele\unc-104^{LZ.UASp.mTagBFP2}/Scer\GAL4^elav.PLu is a non-suppressor of axon | third instar larval stage phenotype of Khc^KO.mutA

(Winding et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The reduced velocity of ooplasmic streaming characteristic for oocytes expressing Khc^GL00330 under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} cannot be rescued by co-expression of Cele\unc-104^{LZ.Scer\UAS.P\T.T:Equa\eqFP578-TagBFP2}.

(Lu et al., 2016)

The reduced axon length observed in cultured neurons from Khc^KO.mutA mutant larvae is not rescued by expression of Cele\unc-104^{LZ.Scer\UAS.P\T.T:Equa\eqFP578-TagBFP2} under the control of Scer\GAL4^elav.PLu in the mutant background.

(Winding et al., 2016)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Cele\unc-104^{LZ.Scer\UAS.P\T.T:Equa\eqFP578-TagBFP2}

Cele\unc-104^{LZ.UASp.TagBFP2}

Cele\unc-104^{LZ.UASp.mTagBFP2}

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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