FlyBase Allele Report: Dmel\wuc[GD6635]

General Information

Symbol

Dmel\wuc^GD6635

Species

D. melanogaster

Name

FlyBase ID

FBal0327605

Feature type

allele

Associated gene

Dmel\wuc

Associated Insertion(s)

Carried in Construct

P{GD6635}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Doggett et al., 2011, Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD6635}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD6635

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

Detailed Description

Statement

Reference

Males expressing wuc^{dsRNA.Scer\UAS} specifically in late spermatogonia and early spermatocytes using Scer\GAL4^{bam.T:Hsim\VP16} show a meiotic arrest testis phenotype when grown at high temperature (30[o]C). The phenotype strength is highly temperature sensitive and there is some evidence of meiosis and early spermatid differentiation when the flies are raised at 29[o]C, significant elongation at 25[o]C and motile sperm at 18[o]C.

(Doggett et al., 2011)

Adults expressing wuc^GD6635 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of tomb^{Scer\UAS.T:Avic\GFP-EGFP} fails to suppress the meiotic arrest testis phenotype found upon expression of wuc^{dsRNA.Scer\UAS} (under the control of Scer\GAL4^{bam.T:Hsim\VP16}).

Co-expression of aly^{Scer\UAS.T:Avic\GFP-EGFP} fails to suppress the meiotic arrest testis phenotype found upon expression of wuc^{dsRNA.Scer\UAS} (under the control of Scer\GAL4^{bam.T:Hsim\VP16}).

Co-expression of mip40^{Scer\UAS.T:Avic\GFP-EGFP} fails to suppress the meiotic arrest testis phenotype found upon expression of wuc^{dsRNA.Scer\UAS} (under the control of Scer\GAL4^{bam.T:Hsim\VP16}).

Co-expression of lin-52^{Scer\UAS.T:Avic\GFP-EGFP} partially suppresses the meiotic arrest testis phenotype found upon expression of wuc^{dsRNA.Scer\UAS} (under the control of Scer\GAL4^{bam.T:Hsim\VP16}), facilitating production of spermatids.

Morphologically, aly⁵ mutants expressing wuc^{dsRNA.Scer\UAS} under the control of Scer\GAL4^{bam.T:Hsim\VP16} are indistinguishable from single mutant testes. Nucleolar disorganisation, as found in aly⁵ mutants, is unaffected by expression of wuc^{dsRNA.Scer\UAS}.

(Doggett et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Co-expression of wuc^{Scer\UAS.T:Avic\GFP-EGFP} rescues the meiotic arrest testis phenotype found upon expression of wuc^{dsRNA.Scer\UAS} (under the control of Scer\GAL4^{bam.T:Hsim\VP16}) such that some post-meiotic differentiation is able to occur, although these flies are still male sterile.

(Doggett et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v45970

w¹¹¹⁸; P{GD6635}v45970

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CG12442^GD6635

wuc^GD6635

wuc^{dsRNA.Scer\UAS}

Name Synonyms

Secondary FlyBase IDs

FBal0199813
FBal0261100

References (6)

Report Sections

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