FB2025_01 , released February 20, 2025
Allele: Dmel\AktT342D.UAS.Tag:HA
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General Information
Symbol
Dmel\AktT342D.UAS.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0343479
Feature type
allele
Associated gene
Dmel\Akt
Associated Insertion(s)
Carried in Construct
P{UAS-Akt.T342D.HA}
Also Known As
UAS-AktT342D
Key Links
Transgenic product class
missense_variant
(Roth et al., 2018)
polypeptide_post_translational_processing_variant
(Roth et al., 2018)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Roth et al., 2018)
polypeptide_post_translational_processing_variant
(Roth et al., 2018)
Mutagen
in vitro construct
(Roth et al., 2018)
Progenitor genotype
Carried in construct
P{UAS-Akt.T342D.HA}
Cytology
Description

UASt regulatory sequences drive expression of full-length Akt1 cDNA (DGRC clone SD10374) containing a phospho-mimicking mutation (T342D), tagged with Tag:HA at the C-terminal.

(Roth et al., 2018)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Akt
Tagged with
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      ameliorates  glucose metabolism disease
      modeled by Tl10b.UAS.cUa
      (Roth et al., 2018)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Clonal expression of Akt1T342D.UAS.Tag:HA under the control of Scer\GAL4Act5C.PP in the larval fat body does not significantly affect nuclei sizes, as compared to neighboring control cells. Expression under the control of Scer\GAL4r4 does not effect the apparent size or weight of pupae, as compared to controls.

      (Roth et al., 2018)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The expression of Tl10b.UAS.cUa under the control of Scer\GAL4r4 leads to smaller pupae with lower weight, whereas clonal expression under the control of Scer\GAL4Act5C.PP in the larval fat body induces induces smaller cells; both cases are substantially suppressed by the co-expression of Akt1T342D.UAS.Tag:HA.

      (Roth et al., 2018)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Akt1T342D.UAS.Tag:HA
      AktT342D.UAS.Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (2)