FB2025_01 , released February 20, 2025
Allele: Dmel\AP-2σang-7
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General Information
Symbol
Dmel\AP-2σang-7
Species
D. melanogaster
Name
FlyBase ID
FBal0344944
Feature type
allele
Associated gene
Dmel\AP-2σ
Associated Insertion(s)
P{EP}AP-2σang-7
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - genetic evidence

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - genetic evidence

(Choudhury et al., 2016)

amorphic allele - molecular evidence

(Choudhury et al., 2016)
Mutagen
Delta2-3 transposase
(Choudhury et al., 2016)
Progenitor genotype
Associated Insertion(s)
P{EP}AP-2σang-7
Cytology
Description

Insertion of a P{EP} element approximately 153bp upstream of lncRNA:Hsrω. Transcription of lncRNA:Hsrω is unaffected, however, there is a severe reduction in the level of transcription of the AP-2σ gene compared to controls.

(Choudhury et al., 2016)
Allele components
Component
Use(s)
Inserted element
P{EP}
Regulatory region(s)
UAS
Mutations Mapped to the Genome
Curation Data
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Whole-eye AP-2σang-7 clones lead to severe morphological defects: small eyes with roughening and loss of bristles; severely disorganized ommatidia of uneven size and with complete loss of photoreceptor cells; and sparse interommatidial bristles. In agreement, these mutant eyes show minimal depolarization in ERGs.

      Larval muscle 6/7 NMJs in AP-2σKG02457/AP-2σang-7, AP-2σang-5/AP-2σang-7 and AP-2σang-2/AP-2σang-7 transheterozygotes show significantly more and significantly smaller boutons, as compared to controls. AP-2σKG02457/AP-2σang-7 and AP-2σang-5/AP-2σang-7 NMJs also show a significant decrease in the vesicle pool and their boutons show a significant decrease in periodic microtubule loop structures (Futsch staining), as compared to controls. Electrophysiology recording at the larval NMJ of AP-2σKG02457/AP-2σang-7 and AP-2σang-5/AP-2σang-7 transheterozygotes also show significant increases in mEJP frequency and amplitude, and significant decreases in EJP amplitude and quantal content, as compared to controls; AP-2σKG02457/AP-2σang-7 and AP-2σang-5/AP-2σang-7 transheterozygotes also show a significant decline in EJP amplitude readings in larval muscles 6/7 upon high-frequency stimulation at 34[o]C conditions, even after a 4min rest period, as compared to controls.

      (Choudhury et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      AP-2σang-7/AP-2σKG02457 is rescued by Scer\GAL4Act5C.PU/AP-2σUAS.cCa

      (Choudhury et al., 2016)
      Partially rescued by

      AP-2σang-7/AP-2σang-5 is partially rescued by Scer\GAL4Act5C.PU/AP-2σUAS.cCa

      (Choudhury et al., 2016)

      AP-2σang-7/AP-2σang-5 is partially rescued by Scer\GAL4elav-C155/AP-2σUAS.cCa

      (Choudhury et al., 2016)

      AP-2σang-7 is partially rescued by AP-2σUAS.cCa/Scer\GAL4ey.PU

      (Choudhury et al., 2016)

      AP-2σang-7/AP-2σKG02457 is partially rescued by Scer\GAL4elav-C155/AP-2σUAS.cCa

      (Choudhury et al., 2016)
      Comments

      The expression of AP-2σUAS.cCa under the control of Scer\GAL4elav-C155 rescues the larval synaptic phenotypes but not the lethality observed in AP-2σKG02457/AP-2σang-7 or AP-2σang-5/AP-2σang-7 transheterozygotes. Expression under the control of Scer\GAL4Act5C.PU rescues both lethality and larval synaptic phenotypes in AP-2σKG02457/AP-2σang-7 transheterozygotes.

      The expression of AP-2σUAS.cCa under the control of Scer\GAL4ey.PU partially rescues the eye morphology and ERG defects induced by AP-2σang-7 whole-eye clones; it fully rescues the photoreceptor cell loss.

      (Choudhury et al., 2016)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      AP-2σang-7
      ang7
      (Choudhury et al., 2016)
      angur7
      (Choudhury et al., 2016)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)