FlyBase Allele Report: Hsap\ROBO1[UAS.cHa]

FB2025_01 , released February 20, 2025

FB2025_01 , released February 20, 2025

Allele: Hsap\ROBO1^UAS.cHa

General Information

Symbol

Hsap\ROBO1^UAS.cHa

Species

H. sapiens

Name

FlyBase ID

FBal0386002

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

PBac{UAS-hROBO1.H}

Key Links

Transgenic product class

(Huang et al., 2022)

(Huang et al., 2022)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

(Huang et al., 2022)

(Huang et al., 2022)

Mutagen

in vitro construct

(Huang et al., 2022)

Progenitor genotype

Carried in construct

PBac{UAS-hROBO1.H}

Cytology

Description

UASt regulatory sequences drive expression of a Hsap\ROBO1 cDNA clone (clone MHS6278-213246291, corresponds to the GB:BC171855.1 isoform). The endogenous stop codon is present, thus even though three copies of the Tag:HA tag are present downstream of the ORF, they are not expected to form part of the translated protein. The human ORF is flanked by a pair of incompatible FRT sites (FRT5 and FRT2), which allows for future in vivo exchange of either the promoter or tag sequence.

(Huang et al., 2022)

Allele components

Component

Use(s)

Regulatory region(s)

binary expression system - regulatory region

Encoded product / tool

Also carries

recombinase target site

recombinase target site

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | embryonic stage, with Scer\GAL4^elav.PLu

(Huang et al., 2022)

abnormal neuroanatomy | embryonic stage, with Scer\GAL4^{robo1-mini-TG4.1}

(Huang et al., 2022)

short lived, with Scer\GAL4^{robo1-mini-TG4.1}

(Huang et al., 2022)

Phenotype Manifest In

connective | embryonic stage, with Scer\GAL4^elav.PLu

(Huang et al., 2022)

connective | embryonic stage, with Scer\GAL4^{robo1-mini-TG4.1}

(Huang et al., 2022)

Detailed Description

Statement

Reference

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Hsap\ROBO1^UAS.cHa/Scer\GAL4^{robo1-mini-TG4.1} is an enhancer of increased mortality during development phenotype of robo1^mini-TG4.1

(Huang et al., 2022)

NOT Suppressor of

Statement

Reference

Hsap\ROBO1^UAS.cHa/Scer\GAL4^{robo1-mini-TG4.1} is a non-suppressor of abnormal neurophysiology | adult stage phenotype of robo1^mini-TG4.1/Df(2R)BSC787

(Huang et al., 2022)

Hsap\ROBO1^UAS.cHa/Scer\GAL4^{robo1-mini-TG4.1} is a non-suppressor of partially lethal - majority die phenotype of robo1^mini-TG4.1/Df(2R)BSC787

(Huang et al., 2022)

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

Hsap\ROBO1^UAS.cHa/Scer\GAL4^{robo1-mini-TG4.1} is a non-suppressor of retina | adult stage phenotype of robo1^mini-TG4.1/Df(2R)BSC787

(Huang et al., 2022)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Hsap\ROBO1^UAS.Tag:HA

Hsap\ROBO1^UAS.cHa

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections