Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast LexA-BD/B42-AD
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.
Source was cell extract of cl8 cell line; bait produced from endogenous gene; prey produced from endogenous gene.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from cell extract of baculovirus infected Sf21 cell line.
Source was cell extract of clone8 cell line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was cell extract of Cl8 cell line; proteins produced from endogenous genes.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast LexA-BD/B42A-AD
Deletion of aa 361 - 390 disrupts the interaction.
Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.
Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.
Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.
F371D,M378D coordinates relative to fu-PA
L380D coordinates relative to fu-PA
F387D coordinates relative to fu-PA
L36D,L41D coordinates relative to Su(fu)-PA
Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.
F371D,M378D coordinates relative to fu-PA
F387D coordinates relative to fu-PA
