FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
hh, boi
FlyBase ID
FBig0000098194
Interaction Network
Interactions Browser links
hh network
boi network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0189929-2.PD.WB
Reference
Yao et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
boi 

Boi

bait

immunogloblulin Fc tag

boi 
hh 

Hh

prey
hh 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
boi 
fibronectin III domain 1
sufficient binding region
hh 
N-terminal region
sufficient binding region

\'HhN\'

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in COS1 cell line; prey derived from conditioned medium of cell line.

(Yao et al., 2006)

hh binds the first FNIII domain of boi, but not its second FNIII domain.

(Yao et al., 2006)
FBrf0209607-7.PD.WB
Reference
Zheng et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
hh 

HhN

bait

GG tag

hh 
boi 

Boi

prey

HA tag

boi 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
hh 
N-terminal region
sufficient binding region
aa 1-257
boi 
fibronectin type III domain 1
necessary binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in unspecified cell line; prey produced as a recombinant fusion protein in S2R+ cell line.

(Zheng et al., 2010)

hh was bound to beads and mixed with detergent-solubilized extracts of transfected S2R+ cell line.

(Zheng et al., 2010)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)