FB2025_01 , released February 20, 2025
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
Pvr, drk
FlyBase ID
FBig0000099072
Interaction Network
Interactions Browser links
Pvr network
drk network
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0180198-2.coIP.WB
Reference
Ishimaru et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
drk 

Drk

bait

FLAG tag

Pvr 

PVR

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Ishimaru et al., 2004)
FBrf0222949-4.Y2H
Reference
Bianco et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Pvr 

PVR

bait
Pvr 
Pvr 
drk 

Drk

prey
drk 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Pvr 
intracellular domain
sufficient binding region
Pvr 
kinase domain
mutation disrupting interaction

kinase dead mutant shows no interaction

drk 
SH2-SH3-SH2 domains
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast

(Bianco et al., 2007)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Bianco et al., 2007)
FBrf0224702-101.coIP.MS
Reference
Friedman et al., 2011
Description
physical association
Assay
Collection
RTK-Ras-ERK_Interactome
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
drk 

drk

bait

TAP tag

Pvr 

Pvr

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Friedman et al., 2011)

Overall SAINT probability of interaction = 1.

(Friedman et al., 2011)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)