Carmon, A., Chien, J., Sullivan, D., Macintyre, R. (2010). The {alpha} Glycerophosphate Cycle in Drosophila melanogaster V. Molecular Analysis of {alpha} Glycerophosphate Dehydrogenase and {alpha} Glycerophosphate Oxidase Mutants.  J. Hered. 101(2): 218--224.

FBrf0210036

Research paper

Two enzymes, alpha glycerophosphate dehydrogenase (GPDH-1) in the cytoplasm and alpha glycerophosphate oxidase (GPO-1) in the mitochondrion cooperate in Drosophila flight muscles to generate the ATP needed for muscle contraction. Null mutants for either enzyme cannot fly. Here, we characterize 15 ethyl methane sulfonate (EMS)-induced mutants in GPDH-1 at the molecular level and assess their effects on structural and evolutionarily conserved domains of this enzyme. In addition, we molecularly characterize 3 EMS-induced GPO-1 mutants and excisions of a P element insertion in the GPO-1 gene. The latter represent the best candidate for null or amorphic mutants in this gene.

PMC2877542 (PMC) (EuropePMC)