FlyBase Insertion Report: Dmel\P{PZ}l(3)05822[05822]

General Information

Symbol

Dmel\P{PZ}l(3)05822⁰⁵⁸²²

Species

D. melanogaster

Name

FlyBase ID

FBti0005544

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

LINE ID

l(3)05822

Affected gene(s)

Ecol\lacZ, l(3)05822

Viability / fertility

Associated allele(s)

Ecol\lacZ⁰⁵⁸²², l(3)05822⁰⁵⁸²²

Stock availability

None publicly available

Genomic Location

Chromosomal Location

3R ( 90F9-90F10 )

Sequence Location

Target / Docking site

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

Orientation

Cytological location (computed by FlyBase)

Cytological location (reported)

90F9-90F10 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

AF006651

(FlyBase, 1992-)

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

Ecol\lacZ

(Harvie et al., 1998, Vosshall, 1998.8.10, BDGP Project Members, 1994-1999)

l(3)05822

(BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ⁰⁵⁸²²

Phenotypic Class Phenotype Manifest in Detailed Description

l(3)05822⁰⁵⁸²²

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

imaginal disc

(Meister and Braun, 1995.10)

eye disc

(Harvie et al., 1998)

ring gland

(Harvie et al., 1998)

embryonic/larval hindgut

(Meister and Braun, 1995.10)

embryonic/larval optic lobe

(Harvie et al., 1998)

larval ventral nerve cord

(Harvie et al., 1998)

adult stage

lamina

(Vosshall, 1998.8.10)

adult antennal lobe

(Vosshall, 1998.8.10)

Additional Information

Statement

Reference

Expression in the larval CNS exhibits a punctate pattern.

(Harvie et al., 1998)

In third instar climbing larva, low level of expression in imaginal discs (which discs not specified), very weak expression in the hindgut.

(Meister and Braun, 1995.10)

Marker for

Reflects expression of

Reporter construct used in assay

Data on Genetic Line

Line ID

l(3)05822

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (0)

External Crossreferences and Linkouts ( 0 )

Comments

This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu).

(FlyBase, 1992-)

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

P1672

(Meister and Braun, 1995.10)

P{PZ}l(3)05822⁰⁵⁸²²

(BDGP Project Members, 1994-1999, FlyBase, 1992-)

P{ry^+t7.2=PZ}l(3)05822⁰⁵⁸²²

(FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

FBti0000598

References (11)

Report Sections

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