FB2025_01 , released February 20, 2025
Insertion: Dmel\P{PZ}ex02278
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General Information
Symbol
Dmel\P{PZ}ex02278
Species
D. melanogaster
Name
FlyBase ID
FBti0005913
Feature type
transposable_element_insertion_site
Description
Inserted Element
P{PZ}
LINE ID

l(2)02278

Affected gene(s)
Ecol\lacZ, ex
Viability / fertility
Associated allele(s)
Ecol\lacZex-02278, ex02278
Stock availability
None publicly available
Genomic Location
Chromosomal Location
2L ( 21C4-21C5 )
Sequence Location
Target / Docking site
Member of Large Scale Dataset(s)
Dataset
TI_set_P{PZ}.BDGP
Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)
Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)
Cytological location (reported)
21C4-21C5 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct
P{PZ}
Size (Kb)

14.545

Component Allele(s)
l(3)87Df7.2
ry+t7.2
Molecular map
Affected Gene(s)
Insertion may affect gene
Ecol\lacZ
(Harvie et al., 1998, BDGP Project Members, 1994-1999)
ex
(BDGP Project Members, 1994-1999)
Variant Molecular Consequences
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
larval stage
corpus allatum
(Harvie et al., 1998)
prothoracic gland
(Harvie et al., 1998)
wandering third instar larval stage
embryonic/larval optic lobe
(Harvie et al., 1998)
larval ventral nerve cord
(Harvie et al., 1998)
Additional Information
Statement
Reference

During all larval stages expressed in lateral secretory cells (prothoracic gland) and medial secretory cells (corpus allatum) of the ring gland.

(Harvie et al., 1998)
 
Marker for
Reflects expression of
ex
(Harvie et al., 1998)
Reporter construct used in assay
Data on Genetic Line
Line ID

l(2)02278

(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (0)
External Crossreferences and Linkouts ( 0 )
Comments
Comments

This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu).

(FlyBase, 1992-)
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
2278
(Harvie et al., 1998)
P{PZ}ex02278
(FlyBase, 1992-)
P{PZ}l(2)0127002278
(BDGP Project Members, 1994-1999, FlyBase, 1992-)
Name Synonyms
Secondary FlyBase IDs
    References (8)