FlyBase Insertion Report: Dmel\P{PZ}hth[06762]

General Information

Symbol

Dmel\P{PZ}hth⁰⁶⁷⁶²

Species

D. melanogaster

Name

FlyBase ID

FBti0006109

Feature type

transposable_element_insertion_site

Also Known As

hth-lacZ

Description

Inserted Element

P{PZ}

LINE ID

l(3)06762

Affected gene(s)

Ecol\lacZ, hth

Viability / fertility

Associated allele(s)

Ecol\lacZ^hth-06762, hth⁰⁶⁷⁶²

Stock availability

None publicly available

Genomic Location

Chromosomal Location

3R ( 86C1-86C2 )

Sequence Location

Target / Docking site

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

Orientation

Cytological location (computed by FlyBase)

Cytological location (reported)

86C1-86C2 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

Ecol\lacZ

(Wernet et al., 2003, Casares and Mann, 2000, Abu-Shaar and Mann, 1998, Rieckhof et al., 1997)

hth

(BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ^hth-06762

Phenotypic Class Phenotype Manifest in Detailed Description

hth⁰⁶⁷⁶²

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

embryonic midgut constriction 1 | posterior to

(Rieckhof et al., 1997)

Malpighian tubule

(Rieckhof et al., 1997)

presumptive embryonic/larval nervous system

(Rieckhof et al., 1997)

embryonic midgut constriction 3 | anterior to

(Rieckhof et al., 1997)

gastric caecum primordium

(Rieckhof et al., 1997)

embryonic stage 7

organism | 10-90% egg length

Comment: 15-85% egg length

(Rieckhof et al., 1997)

embryonic stage 14

endoderm

(Rieckhof et al., 1997)

larval stage

eye-antennal disc

(Rieckhof et al., 1997)

leg disc

(Rieckhof et al., 1997)

wandering third instar larval stage

wing hinge primordium

(Casares and Mann, 2000)

pharate adult stage P8 -- adult stage

dorsal margin photoreceptor cell R7

Comment: expression starts at 45 hr APF

(Wernet et al., 2003)

dorsal margin photoreceptor cell R8

Comment: expression starts at 45 hr APF

(Wernet et al., 2003)

adult stage

medulla | restricted

(Wernet et al., 2003)

Additional Information

Statement

Reference

Expression is observed in maturing inner photoreceptors of the dorsal rim area of the eye. Axons of expressing cells terminate in the dorsal-most part of the medulla, with projections to the R7 and R8 layers.

(Wernet et al., 2003)

The enhancer trap expression pattern is said to be the same as that described for hth.

(Rieckhof et al., 1997)

Marker for

Reflects expression of

hth

(Wernet et al., 2003, Casares and Mann, 2000, Rieckhof et al., 1997)

Reporter construct used in assay

Data on Genetic Line

Line ID

l(3)06762

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (0)

External Crossreferences and Linkouts ( 0 )

Comments

This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu).

(FlyBase, 1992-)

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

P{PZ}hth⁰⁶⁷⁶²

(BDGP Project Members, 1994-1999, FlyBase, 1992-)

hth-lacZ

(Wernet et al., 2003, Casares and Mann, 2000)

hth-lacZ:NLZ

(Wernet et al., 2014)

hth-lacZ:hthP6

(Wang et al., 2016)

hth^l(3)06762-PZ

(Wernet et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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