A Database of Drosophila Genes & Genomes

FB2012_01, released January 20th, 2012
 

Dmel\P{GT1}CerkBG01100 Insertion

General Information
Symbol Dmel\P{GT1}CerkBG01100 Species D. melanogaster
Name FlyBase ID FBti0017197
Feature type transposable_element_insertion_site
Description
Inserted element P{GT1} Expression data
Affected gene(s) Cerk, RpII18 Viability / fertility viable, fertile
Causes allele(s) CerkBG01100 Stock availability 1 publicly available
LINE ID BG01100
Genomic Location
Chromosomal location 3R ( 83A1 ) Sequence location 3R:1,193,320..1,193,320 [+]
Map ( GBrowse ) detailed view FBti0020307 FBti0063263 FBti0053453 FBti0059117 FBti0039623 FBti0068179 FBti0047442 FBti0125912 FBti0050139 FBti0145616 FBti0017197 FBti0037123 FBti0028278 FBti0099989 FBti0055507 FBti0035366 FBti0059074 FBti0037031 FBti0106301 FBti0077079 FBti0069718 FBti0039875 FBti0045510 FBti0126516 FBti0046197 FBti0041796 FBti0105456 FBti0128399 FBti0047058 FBti0037729 FBti0051445
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Description
What does this section display?
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What does this section not display?
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FB2011_10
FB2012_01
References
McElwain et al., 2011, PLoS ONE 6(11): e26993
All updates Click here to see a list of all updates to this record from FB2010_08 and on.
hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
3R:1,193,320..1,193,320 [+]
(Gene Disruption Project members, 2001-)
Orientation
Cytological location
(computed by FlyBase)
83A1 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
83A1 (reported as inferred from sequence location)
(Gene Disruption Project members, 2001-)
Comments concerning
location
hide Sequence Data
Flanking sequence
AZ877676
(Gene Disruption Project members, 2001-, FlyBase, 1992-)
hide Inserted Element
Construct P{GT1}
Location-dependent
role
GAL4 gene trap
(Lukacsovich and Asztalos, 1999.7.15)
Size 8.447, 8.447Kb
Associated alleles
Scer\GAL4GT1
Tn\neoRHsp70Bb.PS
w+mGT
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Cerk
(FlyBase, 1996-, Dasgupta et al., 2009, McElwain et al., 2011)
RpII18
(Gene Disruption Project members, 2001-)
hide Alleles and Phenotypes
Causes alleles
CerkBG01100
(FlyBase, 1996-, Dasgupta et al., 2009, McElwain et al., 2011)
Lethality
References
viable
(Gene Disruption Project members, 2001-)
Sterility
References
fertile
(Gene Disruption Project members, 2001-)
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
Stage 14-16 mutant embryos do not show an increase in mislocalisation of primordial germ cells compared to wild type.
(McElwain et al., 2011)
One copy of Cerk[BG01100] suppresses the maternal effect lethality caused by expression of wntD[Scer\UAS.P\T.cEa] under the control of Scer\GAL4[Act.PU]. Maternal and zygotic double mutant Mulk[LL03414] ; Cerk[BG01100] embryos have an increased average number of mislocalised primordial germ cells compared to wild type at stages 14-16. This phenotype is not seen in either single mutant, or in Mulk[LL03414]/+ ; Cerk[BG01100] mutant embryos carrying a single paternally inherited copy of Mulk[+].
(McElwain et al., 2011)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
BG01100
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
12454
w1118; P{GT1}CerkBG01100
hide Linkouts
hide Comments
Location 3R:1193320-1193321 confirmed by FlyBase alignment of dbGSS accession AZ877676 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
BG01100
(Gene Disruption Project members, 2001-, FlyBase, 1992-)
P{GT1}BG01100
(FlyBase, 1992-, McElwain et al., 2011)
P{GT1}CerkBG01100
 
P{GT1}CG16708BG01100
(FlyBase, 2005, FlyBase, 1992-)
Secondary FlyBase IDs
hide References ( 9 )
Research paper
McElwain et al., 2011, PLoS ONE 6(11): e26993
A suppressor/enhancer screen in Drosophila reveals a role for wnt-mediated lipid metabolism in primordial germ cell migration. [FBrf0216663]
Dasgupta et al., 2009, Proc. Natl. Acad. Sci. U.S.A. 106(47): 20063--20068
Ceramide kinase regulates phospholipase C and phosphatidylinositol 4, 5, bisphosphate in phototransduction. [FBrf0209371]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Supplementary material
Dasgupta et al., 2009, Proc. Natl. Acad. Sci. U.S.A. 106(47):
Supporting information. [FBrf0210341]
Personal communication to FlyBase
Lukacsovich and Asztalos, 1999.7.15, Dual tagging gene-trap of novel genes in Drosophila melanogaster.
Dual tagging gene-trap of novel genes in Drosophila melanogaster. [FBrf0108534]
FlyBase analysis
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1996-, FlyBase inference based on genome sequence analysis.
FlyBase inference based on genome sequence analysis. [FBrf0104946]
FlyBase, 1992-, FlyBase curation
FlyBase curation. [FBrf0105495]
Computer file
Gene Disruption Project members, 2001-, [title not yet available]
[title not yet available] [FBrf0132177]