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General Information
Symbol
Dmel\Mi{PT-GFSTF.1}dpr6MI01358-GFSTF.1
Species
D. melanogaster
Name
FlyBase ID
FBti0178442
Description
Inserted Element
Uses
Affected gene(s)
Viability / fertility
LINE ID
MI01358-GFSTF.1
Genomic Location
Chromosomal Location
3L ( 67D4 )
Sequence Location
3L:10,015,671..10,015,671 [-]
Target / Docking site
Member of Large Scale Dataset(s)
Description
A set of transgenic insertion stocks containing a protein trap cassette, derived from Mi{MIC} insertions in coding introns using Recombinase-Mediated Cassette Exchange (RMCE); see http://flypush.imgen.bcm.tmc.edu/pscreen/technique.html. The protein trap cassette contains a EGFP-FlAsH-StrepII-TEV-3xFlag ("GFSTF") tag that is flanked on either side with a 4X(GlyGlySer) flexible linker; the mini-yellow marker is no longer present. Each insertion line contains one of three versions of the cassette (Mi{PT-GFSTF.0}, Mi{PT-GFSTF.1} or Mi{PT-GFSTF.2}), depending on the reading frame required to generate an in-frame fusion protein with the gene in which the insertion is located. This system also allows UAS/GAL4-based knockdown strategies targeting GFP-sequence containing mRNA (RNAi against GFP) or GFP fusion protein (deGradFP).
Detailed Mapping Data
Chromosome (arm)
Sequence Location
3L:10,015,671..10,015,671 [-]
Cytological location (computed by FlyBase)
67D4 (inferred by FlyBase from sequence location)
Cytological location (reported)
67D4_r6 (reported as inferred from sequence location)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Location-dependent role
Size (Kb)
Associated alleles
Molecular map
Affected Gene(s)
Insertion may affect gene
Alleles and Phenotypes
Expression Data
Reporter Expression
distribution deduced from reporter or direct label
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (1)
External Crossreferences and Linkouts ( 1 )
Crossreferences
Gene Disruption Project - MiMIC RMCE Lines - A collection of protein trap lines made by converting MiMIC insertions by RMCE
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
MI01358 (dpr6)
Mi{PT-GFSTF.1}dpr6MI01358-GFSTF.1
dpr6-EGFP-dpr6
Name Synonyms
Secondary FlyBase IDs
    References (4)