FB2025_01 , released February 20, 2025
Insertion: Dmel\TI{KozakGAL4}AP-1μCR70386-KO-kG4
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General Information
Symbol
Dmel\TI{KozakGAL4}AP-1μCR70386-KO-kG4
Species
D. melanogaster
Name
FlyBase ID
FBti0226918
Feature type
Description
Inserted Element
LINE ID

CR70386

Affected gene(s)
Viability / fertility
Genomic Location
Chromosomal Location
3R ( 85D24 )
Sequence Location
3R:9,557,830..9,559,375 [-]
Target / Docking site
Genomic Maps
Member of Large Scale Dataset(s)
Dataset
Description

A set of stocks in which the coding sequence of a gene of interest has been replaced by a KozakGAL4 promoter trap sequence, resulting in a simultaneous knock-out of the gene of interest and a knock-in of GAL4 driver sequence that is expected to be expressed under the control of the endogenous regulatory sequences of the targeted gene. The cassette was inserted via the technique described in Kanca et al., 2022, eLife 11: e76077 (FBrf0253899).

Detailed Mapping Data
Chromosome (arm)
Sequence Location
3R:9,557,830..9,559,375 [-]
Cytological location (computed by FlyBase)
85D24 (inferred by FlyBase from sequence location)
Cytological location (reported)
85D24_r6 (reported as inferred from sequence location)
Insertion into Natural transposon
Comments concerning location

The upstream end of the deletion associated with the insertion is 385 bp from the annotated TSS of the MBD-like gene on the opposite strand and may affect its expression.

Sequence Data
Flanking sequence
Inserted Element
Size (Kb)
Component Allele(s)
Molecular map
Affected Gene(s)
Variant Molecular Consequences
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Data on Genetic Line
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (1)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
TI{KozakGAL4}AP-1muCR70386-KO-kG4
TI{KozakGAL4}AP-1μCR70386-KO-kG4
Name Synonyms
Secondary FlyBase IDs
    References (1)