A set of stocks in which the coding sequence of a gene of interest has been replaced by a KozakGAL4 promoter trap sequence, resulting in a simultaneous knock-out of the gene of interest and a knock-in of GAL4 driver sequence that is expected to be expressed under the control of the endogenous regulatory sequences of the targeted gene. The cassette was inserted via the technique described in Kanca et al., 2022, eLife 11: e76077 (FBrf0253899).
The upstream end of the deletion associated with the insertion is 385 bp from the annotated TSS of the MBD-like gene on the opposite strand and may affect its expression.