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Targeted recombination event, using P{TV2.FRT(ADGF-A2-.-A-.-B-.w+)} as the donor template. This construct is a donor template for "ends-in" targeted knockout of three genes (Adgf-A2, Adgf-A and Adgf-B). The targeted recombination event is expected to result in a duplication of the three genes, with the expected combination of open reading frames being: Adgf-A2 (wild type), Adgf-A (mutant), Adgf-B (mutant), w (from the donor construct), Adgf-A2 (mutant), Adgf-A (wild type), Adgf-B (wild type). The actual combination of open reading frames is: Adgf-A2 (wild type), Adgf-A (wild type), Adgf-B (mutant), w (from the donor construct), Adgf-A2 (mutant), Adgf-A (wild type), Adgf-B (wild type). This represents a duplication with conversion of mutant Adgf-A to wild type.
Homozygous viable.