Contains a triplication of the Adgf-A2, Adgf-A and Adgf-B genes. The actual combination of open reading frames is: mutant Adgf-A2 containing a 450bp deletion, mutant Adgf-A, mutant Adgf-B, w^+mW.hs marker (inserted during generation of the triplication), mutant Adgf-A2, mutant Adgf-A, mutant Adgf-B, w^+mW.hs marker (inserted during generation of the duplication), mutant Adgf-A2, wild-type Adgf-A, wild-type Adgf-B.

Targeted recombination event, using P{TV2.FRT(ADGF-A2^-.-A^-.-B^-.w⁺)} as the donor template. This construct is a donor template for "ends-in" targeted knockout of three genes (Adgf-A2, Adgf-A and Adgf-B). The targeted recombination event is expected to result in a duplication of the three genes, with the expected combination of open reading frames being: Adgf-A2 (wild type), Adgf-A (mutant), Adgf-B (mutant), w (from the donor construct), Adgf-A2 (mutant), Adgf-A (wild type), Adgf-B (wild type). The actual combination of open reading frames is: Adgf-A2 (wild type), Adgf-A (mutant), Adgf-B (mutant), w (from the donor construct), Adgf-A2 (mutant), Adgf-A (mutant), Adgf-B (mutant), w (from the donor construct), Adgf-A2 (mutant), Adgf-A (wild type), Adgf-B (wild type). This represents a triplication with a 450bp deletion in Adgf-A2.