The distal to proximal progression of the presumptive tarsal segment 5/pretarsus boundary is delayed in some Df(3L)trnΔ17, capsC28fs clones during leg segmentation.
Df(3L)trnΔ17, capsC28fs clones generated by the minute technique cause stunted pretarsal segments and claws in addition to defective joint formation at the tarsal segment 4/tarsal segment 5 and tarsal segment 5/pretarsus boundaries in the adult leg.
Compared to single mutant or wild-type controls, Df(3L)trnΔ17, capsC28fs double mutant clones are more rounded in shape and have smoother boundaries.
The displacement rate of cells from tarsal segment 5 to to the pretarsal segment is further reduced from the 68.8%% in capsC28fs single mutant clones to 26.7% in Df(3L)trnΔ17, capsC28fs double mutants.