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General Information
Symbol
Dmel\E(z)61
Species
D. melanogaster
Name
FlyBase ID
FBal0003440
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
E(z)S2
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

G10635181A

Reported nucleotide change:

G1191A

Amino acid change:

C603Y | E(z)-PA; C603Y | E(z)-PB; C608Y | E(z)-PC

Reported amino acid change:

C603Y

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology

Polytene chromosomes normal.

Nature of the lesion
Statement
Reference

Amino acid replacement: C603Y. Nucleotide substitution: G1191A. Mutation lies within the Cys-rich region which spans amino acids 538-603.

Point mutation.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Homozygous wing and haltere discs are generally smaller than wild type in larvae that have been allowed to complete embryogenesis at the permissive temperature of 18oC and have then been reared at the restrictive temperature of 30oC for 96 hours.

E(z)61 animals derived from eggs laid at 18oC, incubated for an additional 24 hours at 18oC and then transferred to 29oC reach puparium formation and some of them pass through pupal moulting before dying.

Does not cause a small disc phenotype.

Mutation does not affect the level of w expression in ph-plac+3 flies.

Embryos at 29oC display strong posteriorly directed homeotic transformations in which virtually all segments bear eighth abdominal segment-like denticles. Zygotic reduction or loss of trx+ fails to suppress the maternal effect to viability, total loss of zygotic trx+ activity dramatically suppresses maternal effect homeotic transformations.

Homozygous embryos collected over 48 hours at 23oC and then raised at 29oC to the third larval instar show larger, thicker, puffier, less contrasty salivary chromosomes, as if they are decondensed. Chromosome bands are less easy to read because fainter and many characteristic constrictions are absent. Binding of Psc, z and Su(z)2 proteins is dramatically reduced, with remaining binding sites tending to be those that are shared by all three proteins, the z and the polyhomeotic gene products. Psc and Su(z)2 proteins are not lost at the restrictive temperature, just dissociated from the chromatin.

Heterozygous embryos are extremely transformed but there is some phenotypic rescue by abd-A and Abd-B primarily in the head, Abd-B accumulation becomes patchy.

E(z)60/E(z)61 heterozygotes die as pharate adults. At the restrictive temperature embryos from homozygous females die with most body segments transformed towards the eighth abdominal state.

Dominant suppressor of z mutants.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

E(z)32/E(z)61 has visible | homeotic phenotype, enhanceable by Sir2[+]/Sirt105327a

E(z)32/E(z)61 has visible | homeotic phenotype, enhanceable by Sirt1EP2300/Sir2[+]/Scer\GAL4[-]

Suppressed by
Statement
Reference

E(z)731/E(z)61 has increased cell number | male | heat sensitive phenotype, suppressible by Egfrf2

Suppressor of
Statement
Reference

E(z)60/E(z)61 is a suppressor of abnormal eye color phenotype of z1

E(z)61 is a suppressor of abnormal eye color phenotype of z1

Phenotype Manifest In
Suppressed by
Statement
Reference

E(z)731/E(z)61 has testis | heat sensitive phenotype, suppressible by Egfrf2

E(z)731/E(z)61 has cyst progenitor cell | increased number | heat sensitive phenotype, suppressible by Egfrf2

Suppressor of
Statement
Reference

E(z)61 is a suppressor of phenotype of nosunspecified

E(z)61 is a suppressor of phenotype of z1

Additional Comments
Genetic Interactions
Statement
Reference

The formation of ectopic wing veins observed in adult flies expressing Marcal1Scer\UAS.cBa under the control of Scer\GAL4tub.PU is ameliorated by combination with a single copy of E(z)61.

No significant modification of the number of sex combs is seen in E(z)61/+ corto420/+ double heterozygotes compared to E(z)61/+ single heterozygotes.

E(z)61/E(z)61 shows 6% suppression of the nos abdominal phenotype at 29oC, and 2% at 25oC. Suppression is enhanced to 38% (at 29oC), and 24% (at 25oC) for E(z)61/E(z)65, and to 95% for E(z)son2/E(z)61.

Temperature sensitive recessive suppressor of the z1 eye colour.

E(z)60/E(z)61 heterozygotes display extreme suppression of the z1 eye colour.

Xenogenetic Interactions
Statement
Reference

The formation of ectopic wing veins observed in adult flies expressing Hsap\SMARCAL1Scer\UAS.cBa under the control of Scer\GAL4Bx-MS1096 is ameliorated by combination with a single copy of E(z)61.

Complementation and Rescue Data
Comments

One maternal copy of P{FLAG-E(z).W} completely rescues the maternal effect embryonic lethality of E(z)61.

E(z)+t8.8 fully rescues hemizygous lethality and the maternal effect embryonic lethality of E(z)61/Df(3L)lxd6 reared at 29oC.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

Mutation is null in regard to homeotic function at the restictive temperature 29oC (Jones, Genetics 126: 185--199). By 9 hours abd-A is expressed throughout the A-P axis including the head, Abd-B is expressed throughout the axis in nearly all epidermal cells.

Silencing of Scer\GAL4-BXD genes is dependent on Pc group gene function, in mutant embryos silencing is lost.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
References (38)