FlyBase Allele Report: Dmel\E(z)[61]

General Information

Symbol

Dmel\E(z)⁶¹

Species

D. melanogaster

Name

FlyBase ID

FBal0003440

Feature type

allele

Associated gene

Dmel\E(z)

Associated Insertion(s)

Carried in Construct

Also Known As

E(z)^S2

Key Links

Genomic Maps

JBrowse

Allele class

heat sensitive loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

heat sensitive loss of function allele

(Simon et al., 1992)

Mutagen

ethyl methanesulfonate

(Jones and Gelbart, 1990)

Progenitor genotype

Cytology

Polytene chromosomes normal.

(Jones and Gelbart, 1990)

Description

Amino acid replacement: C603Y. Nucleotide substitution: G1191A. Mutation lies within the Cys-rich region which spans amino acids 538-603.

(Carrington and Jones, 1996)

Point mutation.

(Platero et al., 1996)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:10,635,181..10,635,181 [-]

Nucleotide change:

G10635181A

Reported nucleotide change:

G1191A

Amino acid change:

C603Y | E(z)-PA; C603Y | E(z)-PB; C608Y | E(z)-PC

Reported amino acid change:

C603Y

(Carrington and Jones, 1996)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell number | male | heat sensitive (with E(z)⁷³¹)

(Eun et al., 2014)

increased occurrence of cell division | male | heat sensitive (with E(z)⁷³¹)

(Eun et al., 2014)

lethal | heat sensitive

(Zhimulev et al., 2003, LaJeunesse and Shearn, 1996, Kuzin et al., 1994, Simon et al., 1992)

lethal | recessive

lethal | recessive | heat sensitive

(Rastelli et al., 1993)

lethal - all die during embryonic stage | heat sensitive

(Wu et al., 1989)

some die during embryonic stage | heat sensitive

(Wu et al., 1989)

viable (with E(z)³²)

(Furuyama et al., 2004)

visible | homeotic (with E(z)³²)

(Furuyama et al., 2004)

visible | recessive | heat sensitive

(Jones and Gelbart, 1993)

Phenotype Manifest In

cyst progenitor cell | increased number | heat sensitive (with E(z)⁷³¹)

(Eun et al., 2014)

haltere disc

(Papp and Muller, 2006)

male germline cell | heat sensitive (with E(z)⁷³¹)

(Eun et al., 2014)

polytene chromosome | heat sensitive

(Rastelli et al., 1993)

segment | heat sensitive

(Jones and Gelbart, 1993)

testis | heat sensitive (with E(z)⁷³¹)

(Eun et al., 2014)

wing disc

(Papp and Muller, 2006)

Detailed Description

Statement

Reference

Homozygous wing and haltere discs are generally smaller than wild type in larvae that have been allowed to complete embryogenesis at the permissive temperature of 18^oC and have then been reared at the restrictive temperature of 30^oC for 96 hours.

(Papp and Muller, 2006)

E(z)⁶¹ animals derived from eggs laid at 18^oC, incubated for an additional 24 hours at 18^oC and then transferred to 29^oC reach puparium formation and some of them pass through pupal moulting before dying.

(Zhimulev et al., 2003)

Does not cause a small disc phenotype.

(LaJeunesse and Shearn, 1996)

Mutation does not affect the level of w expression in ph-p^lac+3 flies.

(Fauvarque et al., 1995)

Embryos at 29^oC display strong posteriorly directed homeotic transformations in which virtually all segments bear eighth abdominal segment-like denticles. Zygotic reduction or loss of trx⁺ fails to suppress the maternal effect to viability, total loss of zygotic trx⁺ activity dramatically suppresses maternal effect homeotic transformations.

(Jones and Gelbart, 1993)

Homozygous embryos collected over 48 hours at 23^oC and then raised at 29^oC to the third larval instar show larger, thicker, puffier, less contrasty salivary chromosomes, as if they are decondensed. Chromosome bands are less easy to read because fainter and many characteristic constrictions are absent. Binding of Psc, z and Su(z)2 proteins is dramatically reduced, with remaining binding sites tending to be those that are shared by all three proteins, the z and the polyhomeotic gene products. Psc and Su(z)2 proteins are not lost at the restrictive temperature, just dissociated from the chromatin.

(Rastelli et al., 1993)

Heterozygous embryos are extremely transformed but there is some phenotypic rescue by abd-A and Abd-B primarily in the head, Abd-B accumulation becomes patchy.

(Simon et al., 1992)

E(z)⁶⁰/E(z)⁶¹ heterozygotes die as pharate adults. At the restrictive temperature embryos from homozygous females die with most body segments transformed towards the eighth abdominal state.

(Wu et al., 1989)

Dominant suppressor of z mutants.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

E(z)³²/E(z)⁶¹ has visible | homeotic phenotype, enhanceable by Sir2[+]/Sirt1^05327a

(Furuyama et al., 2004)

E(z)³²/E(z)⁶¹ has visible | homeotic phenotype, enhanceable by Sirt1^EP2300/Sir2[+]

(Furuyama et al., 2004)

Suppressed by

Statement

Reference

E(z)⁷³¹/E(z)⁶¹ has increased cell number | male | heat sensitive phenotype, suppressible by Egfr^f2

(Eun et al., 2014)

Suppressor of

Statement

Reference

E(z)[+]/E(z)⁶¹ is a suppressor of visible | adult stage phenotype of Marcal1^UAS.cBa, Scer\GAL4^Tub.PU

(Morimoto et al., 2016)

E(z)[+]/E(z)⁶¹ is a suppressor of visible | adult stage phenotype of Hsap\SMARCAL1^UAS.cBa, Scer\GAL4^Bx-MS1096

(Morimoto et al., 2016)

E(z)⁶⁰/E(z)⁶¹ is a suppressor of abnormal eye color phenotype of z¹

(Wu et al., 1989)

E(z)⁶¹ is a suppressor of abnormal eye color phenotype of z¹

Phenotype Manifest In

Suppressed by

Statement

Reference

E(z)⁷³¹/E(z)⁶¹ has cyst progenitor cell | increased number | heat sensitive phenotype, suppressible by Egfr^f2

(Eun et al., 2014)

E(z)⁷³¹/E(z)⁶¹ has testis | heat sensitive phenotype, suppressible by Egfr^f2

(Eun et al., 2014)

Suppressor of

Statement

Reference

E(z)[+]/E(z)⁶¹ is a suppressor of wing vein | adult stage phenotype of Marcal1^UAS.cBa, Scer\GAL4^Tub.PU

(Morimoto et al., 2016)

E(z)[+]/E(z)⁶¹ is a suppressor of wing vein | adult stage phenotype of Hsap\SMARCAL1^UAS.cBa, Scer\GAL4^Bx-MS1096

(Morimoto et al., 2016)

E(z)⁶¹ is a suppressor of phenotype of nanos^unspecified

(Pelegri and Lehmann, 1994)

E(z)⁶¹ is a suppressor of phenotype of z¹

(Jones and Gelbart, 1990)

Additional Comments

Genetic Interactions

Statement

Reference

The formation of ectopic wing veins observed in adult flies expressing Marcal1^Scer\UAS.cBa under the control of Scer\GAL4^tub.PU is ameliorated by combination with a single copy of E(z)⁶¹.

(Morimoto et al., 2016)

No significant modification of the number of sex combs is seen in E(z)⁶¹/+ corto⁴²⁰/+ double heterozygotes compared to E(z)⁶¹/+ single heterozygotes.

(Lopez et al., 2001)

E(z)⁶¹/E(z)⁶¹ shows 6% suppression of the nos abdominal phenotype at 29^oC, and 2% at 25^oC. Suppression is enhanced to 38% (at 29^oC), and 24% (at 25^oC) for E(z)⁶¹/E(z)⁶⁵, and to 95% for E(z)^son2/E(z)⁶¹.

(Pelegri and Lehmann, 1994)

Temperature sensitive recessive suppressor of the z¹ eye colour.

(Jones and Gelbart, 1990)

E(z)⁶⁰/E(z)⁶¹ heterozygotes display extreme suppression of the z¹ eye colour.

(Wu et al., 1989)

Xenogenetic Interactions

Statement

Reference

The formation of ectopic wing veins observed in adult flies expressing Hsap\SMARCAL1^Scer\UAS.cBa under the control of Scer\GAL4^Bx-MS1096 is ameliorated by combination with a single copy of E(z)⁶¹.

(Morimoto et al., 2016)

Complementation and Rescue Data

Rescued by

E(z)⁶¹ is rescued by E(z)^Tag:FLAG

(Wang et al., 2002)

Df(3L)lxd6/E(z)⁶¹ is rescued by E(z)^+t8.8

(Jones and Gelbart, 1993)

E(z)⁶¹ is rescued by E(z)^+t8.8

(Jones and Gelbart, 1993)

Comments

One maternal copy of P{FLAG-E(z).W} completely rescues the maternal effect embryonic lethality of E(z)⁶¹.

(Wang et al., 2002)

E(z)^+t8.8 fully rescues hemizygous lethality and the maternal effect embryonic lethality of E(z)⁶¹/Df(3L)lxd6 reared at 29^oC.

(Jones and Gelbart, 1993)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Mutation is null in regard to homeotic function at the restictive temperature 29^oC (Jones, Genetics 126: 185--199). By 9 hours abd-A is expressed throughout the A-P axis including the head, Abd-B is expressed throughout the axis in nearly all epidermal cells.

(Simon et al., 1992)

Silencing of Scer\GAL4-BXD genes is dependent on Pc group gene function, in mutant embryos silencing is lost.

(Muller, 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

E(z)-TS

(Zenk et al., 2017)

E(z)61

(Gvozdev et al., 2000, Naumova et al., 2000)

E(z)⁶¹

(Kang et al., 2022, Ghotbi et al., 2020, Zenk et al., 2017, Morimoto et al., 2016, Kang et al., 2015, Eun et al., 2014, Herz et al., 2012, Fujioka et al., 2008, Goodliffe et al., 2007, Papp and Muller, 2006, Kalmykova et al., 2005, Ketel et al., 2005)

E(z)^S2

(Perrini et al., 2004, Zhimulev et al., 2003, Czermin et al., 2002, Chan et al., 1994, Kuzin et al., 1994, Rastelli et al., 1993, Simon et al., 1993, Wu et al., 1989)

E(z)^s2

(Muller, 1995)

E(z)S2

(Jones and Gelbart, 1990)

Name Synonyms

Secondary FlyBase IDs

References (42)

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