Homozygous females have a grandchildless phenotype with a penetrance of 88% at 25oC.
Embryos derived from homozygous females show reduced nuclear migration throughout the cleavage stages, with no posterior migration detected at cycle 2. The orientation of the two sister nuclei with respect to the antero-posterior axis at this stage is random, in contrast to wild-type.
Embryos derived from homozygous gs(1)N411 females raised at the restrictive temperature (25oC) had hardly any space under the vitelline membrane at the posterior pole throughout the cleavage stage. Nuclear arrival in the posterior pole periplasm at the cleavage stage is delayed by 30 minutes compared with nuclear arrival in other regions. This is due to delayed migration of the nuclei. F-actin organisation is abnormal at the cleavage stage.
Fecundity and fertility of homozygous females normal. 71% and 19% agametic progeny when females raised at 25oC and 18oC, respectively. Peripheral migration of nuclei at blastoderm formation normal; pole cell formation inhibited. Temperature sensitive period extends from stages 9-13 of oocyte development.
