Amino acid replacement: W90T.
In addition to the W90T amino acid replacement, which may cause the mutant phenotype, a number of additional amino acid changes are present compared to the U34925 mei-41 GenBank sequence (these "common" mutations are also present in other mei-41 mutant alleles, possibly due to variability present in the mutagenised population).
Nucleotide substitution: T2585A.
T16392576A
T2585A
W253R | mei-41-PA
W90T
The mutation site reported in FBrf0160718 is relative to GB:U34925 (in which the predicted CDS is missing 163 N-terminal amino acids relative to genome release 3.2). The reported nucleotide change predicts a W to R change rather than W to T as reported. Other amino acid changes common to several mei-41 mutants are also present in strain (see FBrf0160718).
C16398915T
C8924T
P2322L | mei-41-PA
P2159L
The mutation site reported in FBrf0160718 is relative to GB:U34925 (in which the predicted CDS is missing 163 N-terminal amino acids relative to genome release 3.2). Other amino acid changes common to several mei-41 mutants are also present in the strain (see FBrf0160718).
Wild-type embryos show an increase in the ratio of metaphase to (anaphase+telophase) 40 minutes after irradiation, compared to unirradiated controls. This increase is partially diminished in homozygous embryos derived from homozygous females. 98% of eggs derived from homozygous females hatch into larvae. Hemizygous larvae show 35% loss of the G2/M checkpoint (this number is the average number of mitotic cells per eye disc after exposure of male hemizygous larvae to 500R of X rays expressed as a percentage of the number of mitotic cells per eye disc before irradiation, wild-type values range from 5 to 15%).
Does not reduce meiotic exchange.
