FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Dna2D1
Open Close
General Information
Symbol
Dmel\Dna2D1
Species
D. melanogaster
Name
FlyBase ID
FBal0012614
Feature type
allele
Associated gene
Dmel\Dna2
Associated Insertion(s)
Carried in Construct
Also Known As
mus109D1
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
Progenitor genotype
Cytology
Description

Amino acid replacement: E500D.

(Mitchell et al., 2022)

Contains eight missense mutations, all of which likely affect the functionality of the Dna2 gene product, followed by a nonsense mutation in the 'helicase 2A' domain at codon L871.

(Mitchell et al., 2022)

Amino acid replacement: L512F.

(Mitchell et al., 2022)

Amino acid replacement: S571G.

(Mitchell et al., 2022)

Amino acid replacement: E572Q.

(Mitchell et al., 2022)

Amino acid replacement: I633V.

(Mitchell et al., 2022)

Amino acid replacement: C755S.

(Mitchell et al., 2022)

Amino acid replacement: E825A.

(Mitchell et al., 2022)

Amino acid replacement: K826E.

(Mitchell et al., 2022)

Amino acid replacement: L871term.

(Mitchell et al., 2022)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
X:10,012,127..10,012,127 [+]
Nucleotide change:

G10012127Y

Amino acid change:

E500D | Dna2-PB; E499D | Dna2-PC

Reported amino acid change:

E500D

Comment:

Site and nature of nucleotide substitution in mutant inferred by FlyBase curator based on reported amino acid change.

(Mitchell et al., 2022)
point_mutation
X:10,012,163..10,012,163 [+]
Nucleotide change:

G10012163Y

Amino acid change:

L512F | Dna2-PB; L511F | Dna2-PC

Reported amino acid change:

L512F

Comment:

Site and nature of nucleotide substitution in mutant inferred by FlyBase curator based on reported amino acid change.

(Mitchell et al., 2022)
point_mutation
X:10,012,397..10,012,397 [+]
Nucleotide change:

A10012397G

Amino acid change:

S571G | Dna2-PB; S570G | Dna2-PC

Reported amino acid change:

S571G

Comment:

Site and nature of nucleotide substitution in mutant inferred by FlyBase curator based on reported amino acid change.

(Mitchell et al., 2022)
point_mutation
X:10,012,400..10,012,400 [+]
Nucleotide change:

G10012400C

Amino acid change:

E572Q | Dna2-PB; E571Q | Dna2-PC

Reported amino acid change:

E572Q

Comment:

Site and nature of nucleotide substitution in mutant inferred by FlyBase curator based on reported amino acid change.

(Mitchell et al., 2022)
point_mutation
X:10,012,583..10,012,583 [+]
Nucleotide change:

A10012583G

Amino acid change:

I633V | Dna2-PB; I632V | Dna2-PC

Reported amino acid change:

I633V

Comment:

Site and nature of nucleotide substitution in mutant inferred by FlyBase curator based on reported amino acid change.

(Mitchell et al., 2022)
point_mutation
X:10,012,949..10,012,949 [+]
Nucleotide change:

T10012949A

Amino acid change:

C755S | Dna2-PB; C754S | Dna2-PC

Reported amino acid change:

C755S

Comment:

A nucleotide change at the first (T to A) or second (G to C) position of the Cys codon leads to a Ser mutation (base change unspecified). Site and nature of nucleotide substitution in mutant inferred by FlyBase curator based on reported amino acid change.

(Mitchell et al., 2022)
point_mutation
X:10,013,160..10,013,160 [+]
Nucleotide change:

A10013160C

Amino acid change:

E825A | Dna2-PB; E824A | Dna2-PC

Reported amino acid change:

E825A

Comment:

Site and nature of nucleotide substitution in mutant inferred by FlyBase curator based on reported amino acid change.

(Mitchell et al., 2022)
point_mutation
X:10,013,162..10,013,162 [+]
Nucleotide change:

A10013162G

Amino acid change:

K826E | Dna2-PB; K825E | Dna2-PC

Reported amino acid change:

K826E

Comment:

Site and nature of nucleotide substitution in mutant inferred by FlyBase curator based on reported amino acid change.

(Mitchell et al., 2022)
point_mutation
X:10,013,298..10,013,298 [+]
Nucleotide change:

T10013298A

Amino acid change:

L871term | Dna2-PB; L870term | Dna2-PC

Reported amino acid change:

L871term

Comment:

Site and nature of nucleotide substitution in mutant inferred by FlyBase curator based on reported amino acid change.

(Mitchell et al., 2022)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      mitosis & nuclear chromosome

      (Gatti, 1979)
      Detailed Description
      Statement
      Reference

      Dna2D1/Dna2D1, Dna2D1/Dna2D2 and Dna2D1/Dna2lS individuals show increased sensitivity to the mutagen MMS.

      (Mitchell et al., 2022)

      mus109D1 mutants have a similar frequency of single-strand annealing repair (SSA) compared to controls in a P{wIw.FRT} hemizygous assay to study DNA double-stranded break repair when assayed at 32oC or 38oC.

      (Wei and Rong, 2007)

      Homozygotes and hemizygotes show a higher frequency of spontaneous chromosome aberrations in neuroblast metaphases than wild-type larvae. The ratio of chromatid breaks to isochromatid breaks is 0.7-0.8. Approximately 80% of the breaks are heterochromatic. The breaks appear to be randomly distributed among the chromosomes. The frequency of breaks is the same in females and males.

      (Gatti, 1979)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to complement

      Dna2unspecified

      (Gatti, 1979)
      Comments

      Fails to complement mus109unspecified for female sterility.

      (Gatti, 1979)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer

      Boyd.

      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (5)
      Reported As
      Symbol Synonym
      Dna2D1
      mus(1)107D1
      (Olsen and Green, 1982)
      mus-109D1
      (Gatti, 1979)
      mus107D1
      mus109D1
      (Mitchell et al., 2022, Wei and Rong, 2007)
      Name Synonyms
      Secondary FlyBase IDs
        References (6)