FlyBase Allele Report: Dmel\ref(2)P[p]

General Information

Symbol

Dmel\ref(2)P^p

Species

D. melanogaster

Name

FlyBase ID

FBal0014525

Feature type

allele

Associated gene

Dmel\ref(2)P

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

natural population

Nature of the Allele

Allele class

Mutagen

natural population

(Wayne et al., 1996, Laboratoire de Genetique Formelle du C.N.R.S., 1953)

Progenitor genotype

Cytology

Description

DNA sequence of ref(2)P^p and ref(2)P^o1 have been compared, revealing numerous point mutations all along the sequence. Four main variations are (i) In exon 1, ref(2)P^o1 encodes glutamine-asparagine (nucleotides 453-458 read CAGAAT), ref(2)P^p encodes glycine (nucleotides GGA), (ii) In exon 2, ref(2)P^p has a 21bp deletion between positions 1556 and 1578, (iii) In exon 2, ref(2)P^p has a 24bp deletion between positions 2196 and 2221, (iv) In intron 1 of ref(2)P^p a 7bp insertion is a perfect repeat of a motif that may be important in secondary structure and is present 23bp upstream.

(Dru et al., 1993)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

sequence_variant

2L:19,542,898..19,542,903 [+]

Comment:

Replaced with "GGA" in ref(2)P^p strain which is restrictive for sigma virus replication. One of several differences between permissive ref(2)P^o and restrictive ref(2)P^p strains.

(Dru et al., 1993)

sequence_variant

2L:19,542,910..19,542,910 [+]

Nucleotide change:

A19538839G

Amino acid change:

I32V | ref(2)P-PA; I32V | ref(2)P-PB

Comment:

One of several differences between permissive ref(2)P^o and restrictive ref(2)P^p strains.

(Dru et al., 1993)

sequence_variant

2L:19,542,944..19,542,944 [+]

Nucleotide change:

A19538873T

Amino acid change:

Q43L | ref(2)P-PA; Q43L | ref(2)P-PB

Comment:

One of several differences between permissive ref(2)P^o and restrictive ref(2)P^p strains.

(Dru et al., 1993)

sequence_variant

2L:19,543,051..19,543,051 [+]

Comment:

Insertion of "TGTTTGC" in ref(2)P^p strain which is restrictive for sigma virus replication. One of several differences between permissive ref(2)P^o and restrictive ref(2)P^p strains.

(Dru et al., 1993)

sequence_variant

2L:19,544,002..19,544,022 [+]

Comment:

Deletion of 21bp in ref(2)P^p strain which is restrictive for sigma virus replication. One of several differences between permissive ref(2)P^o and restrictive ref(2)P^p strains.

(Dru et al., 1993)

sequence_variant

2L:19,544,138..19,544,138 [+]

Nucleotide change:

A19540067T

Amino acid change:

T231S | ref(2)P-PA; T231S | ref(2)P-PB

Comment:

One of several differences between permissive ref(2)P^o and restrictive ref(2)P^p strains.

(Dru et al., 1993)

sequence_variant

2L:19,544,438..19,544,438 [+]

Nucleotide change:

A19540367C

Amino acid change:

S331R | ref(2)P-PA; S331R | ref(2)P-PB

Comment:

One of several differences between permissive ref(2)P^o and restrictive ref(2)P^p strains.

(Dru et al., 1993)

sequence_variant

2L:19,544,647..19,544,670 [+]

Comment:

Deletion of 24bp in ref(2)P^p strain which is restrictive for sigma virus replication. One of several differences between permissive ref(2)P^o and restrictive ref(2)P^p strains.

(Dru et al., 1993)

sequence_variant

2L:19,545,073..19,545,073 [+]

Nucleotide change:

C19541002G

Amino acid change:

D542E | ref(2)P-PA; D542E | ref(2)P-PB

Comment:

One of several differences between permissive ref(2)P^o and restrictive ref(2)P^p strains.

(Dru et al., 1993)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Restrictive for sigma virus multiplication. CO₂ resistant.

(Dru et al., 1993)

Restrictive for rhabovirus sigma infection.

(Contamine et al., 1989)

RK1. No known morphological effect. Growth of CO₂-sensitivity virus is inhibited when in homozygous state.

(Laboratoire de Genetique Formelle du C.N.R.S., 1953)

restrictive allele

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Isolated from: Paris stock.

(Laboratoire de Genetique Formelle du C.N.R.S., 1953)

Comments

Frequency of ref(2)P^p allele compared to that of ref(2)P^o did not change during time course of study of sigma virus Type I and Type II frequencies.

(Fleuriet and Sperlich, 1992)

Restrictive (resistant) line to the sigma virus, determined by a CO₂ sensitivity assay.

(Wayne et al., 1996)

Reduces the possibility of infection and prevents hereditary transmission. Found in Paris strain.

(Gay, 1972)

External Crossreferences and Linkouts ( 2 )

Crossreferences

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

X69828

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

CAA49482

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

ref(2)P^(dpp')Pa

(Carre-Mlouka et al., 2007)

ref(2)P^P

(Fleuriet and Sperlich, 1992)

ref(2)P^p

(Carre-Mlouka et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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