FlyBase Allele Report: Dmel\sro[1]

General Information

Symbol

Dmel\sro¹

Species

D. melanogaster

Name

FlyBase ID

FBal0016078

Feature type

allele

Associated gene

Dmel\sro

Associated Insertion(s)

Carried in Construct

Also Known As

kay^sro-1

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Tearle and Nusslein-Volhard, 1987)

Progenitor genotype

Cytology

Description

Nucleotide substitution: C?T.

(Niwa et al., 2010)

Amino acid replacement: Q132term.

(Niwa et al., 2010)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:29,795,084..29,795,084 [+]

Nucleotide change:

C29795084T

Reported nucleotide change:

C?T

Amino acid change:

Q132term | sro-PA

Reported amino acid change:

Q132term

(Niwa et al., 2010)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Niwa et al., 2010)

lethal - all die during embryonic stage (with sro^O4-105)

(Niwa et al., 2010)

lethal - all die during embryonic stage | recessive

(Hudson and Goldstein, 2008)

some die during embryonic stage (with sro^O4-105)

(Niwa et al., 2010)

some die during embryonic stage | recessive

(Hudson and Goldstein, 2008)

Phenotype Manifest In

adult cuticle

cephalopharyngeal skeleton

embryo | dorsal closure stage

(Chavez et al., 2000)

embryonic/first instar larval cuticle

(Niwa et al., 2010, Chavez et al., 2000)

midgut primordium

(Chavez et al., 2000)

Detailed Description

Statement

Reference

Homozygous embryos have undifferentiated cuticle.

sro¹/sro^O4-105 animals normally die as embryos, but if they are treated with 20-hydroxyecdysone midway through embryogenesis, approximately 52% hatch into first instar larvae. Approximately 45% of these rescued animals grow into second instar larvae if they are raised on a diet supplemented with 20-hydroxyecdysone.

(Niwa et al., 2010)

Lethality occurs before embryonic stage 8.

(Hudson and Goldstein, 2008)

7 to 16 hour homozygous embryos contain relatively normal concentrations of both ecdysone and 20-hydroxyecdysone compared to control embryos. Mutants fail to make cuticle and show defects in morphogenetic movements during late embryogenesis that lead to a failure of head involution and defective midgut formation.

(Chavez et al., 2000)

Larval mouth parts and cuticle not differentiated.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

sro¹ has lethal | recessive phenotype, suppressible by Bmor\nm-g^UAS.cNa/Scer\GAL4^2-286

(Niwa et al., 2010)

NOT Enhancer of

Statement

Reference

sro¹ is a non-enhancer of visible phenotype of Ppt1^UAS.cKa, Scer\GAL4^GMR.PF

(Buff et al., 2007)

NOT Suppressor of

Statement

Reference

sro¹ is a non-suppressor of visible phenotype of Ppt1^UAS.cKa, Scer\GAL4^GMR.PF

(Buff et al., 2007)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

sro¹ is a non-enhancer of eye phenotype of Ppt1^UAS.cKa, Scer\GAL4^GMR.PF

(Buff et al., 2007)

NOT Suppressor of

Statement

Reference

sro¹ is a non-suppressor of eye phenotype of Ppt1^UAS.cKa, Scer\GAL4^GMR.PF

(Buff et al., 2007)

sro¹ is a non-suppressor of phenotype of arm³

(Cox et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

At 22[o]C, Scer\GAL4^en-e16E>kay^{dsRNA.Scer\UAS} expression does not affect adult wing size in a wild-type background but the size of the posterior compartment is reduced when this transgene is expressed in a sro¹/+ background.

(Hyun et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

sro¹ is rescued by Scer\GAL4^2-286/sro^UAS.cNa

(Niwa et al., 2010)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

3091

106834

Notes on Origin

Discoverer

Comments

sro¹ complements kay¹, kay^S135103, kay^EY01644 and kay^EY08232.

(Hudson and Goldstein, 2008)

sro¹ complements kay¹.

(Niwa et al., 2010)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

Kay^Sro

(Casas-Tinto et al., 2008)

Sro¹

(Talsma et al., 2014)

kay^8A

kay^sro-1

(Christensen et al., 2008.6.11, Buff et al., 2007, Hyun et al., 2006)

kay^sro1

(Pearson et al., 2009)

sro-1

(Hudson and Goldstein, 2008)

sro¹

(Niwa et al., 2010, Chavez et al., 2000, Cox et al., 2000)

sro^8A34

(Harbecke and Lengyel, 1995)

sro^8A

(Tearle and Nusslein-Volhard, 1987)

Name Synonyms

shroud1

(Rewitz et al., 2010)

Secondary FlyBase IDs

References (16)

Report Sections

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