FlyBase Allele Report: Dmel\up[int-3]

General Information

Symbol

Dmel\up^int-3

Species

D. melanogaster

Name

FlyBase ID

FBal0017636

Feature type

allele

Associated gene

Dmel\up

Associated Insertion(s)

Carried in Construct

Also Known As

int³

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Deak et al., 1982)

Progenitor genotype

Cytology

Description

Amino acid replacement: E100K. Nucleotide substitution: G?A.

(Fyrberg et al., 1990)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:13,596,540..13,596,540 [-]

Nucleotide change:

G13596540A

Reported nucleotide change:

GAG?AAG

Amino acid change:

Reported amino acid change:

E100K

Comment:

Position of mutation on reference sequence inferred by FlyBase curator based on author statement. See FBrf0052459 figure 3 for protein sequence numbering.

(Fyrberg et al., 1990)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

flightless

(Deak et al., 1982)

flightless | recessive

(Homyk and Emerson, 1988)

visible | recessive

(Homyk and Emerson, 1988)

Phenotype Manifest In

indirect flight muscle cell

(Barthmaier and Fyrberg, 1995)

sarcomere | adult stage

(Fyrberg et al., 1990)

wing

(Homyk and Emerson, 1988)

Detailed Description

Statement

Reference

Flight muscles develop nearly normally and then degenerate during early adulthood when fibres become contractile.

(Barthmaier and Fyrberg, 1995)

Myofibril structure appears normal prior to the onset of flight-muscle contraction in homozygous pupae, however, after the fly ecloses sarcomeric structure rapidly degenerates.

(Fyrberg et al., 1990)

Homozygotes are flightless, and have an abnormal wing posture.

(Homyk and Emerson, 1988)

Flies are flightless and hold their wings in an abnormal position, and sometimes show variable jump ability.

(Deak et al., 1982)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Mhc⁵, up^int-3 has visible | dominant phenotype

(Homyk and Emerson, 1988)

Mhc⁵, up^int-3 has flightless | dominant phenotype

(Homyk and Emerson, 1988)

Mhc⁶, up^int-3 has flightless | dominant phenotype

(Homyk and Emerson, 1988)

Mhc⁸, up^int-3 has lethal | dominant phenotype

(Homyk and Emerson, 1988)

up^int-3, wupA^hdp-2 has visible | dominant phenotype

(Homyk and Emerson, 1988)

up^int-3, wupA^hdp-2 has flightless | dominant phenotype

(Homyk and Emerson, 1988)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

up^int-3 Mhc⁸ double heterozygotes are lethal. Female up^int-3 Mhc⁵ double heterozygotes are completely flightless and have an abnormal wing posture. Male up^int-3 heterozygotes also hemizygous for Mhc⁵ have reduced viability, the rare escapers have "gnarled" legs, walk poorly, and die within 2 days of eclosion. Double heterozygotes with Mhc⁶ are flightless. Double heterozygotes with wupA^hdp-2 are flightless and have an abnormal wing posture. Double heterozygotes with wupA^hdp-101 or fliH¹ have greatly reduced flight ability. Most double heterozygotes with wupA^hdp-4, wupA^hdp-3 or wupA^hdp-5 have an abnormal wing posture.

(Homyk and Emerson, 1988)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

int³

(Vigoreaux, 2001, Fyrberg et al., 1990, Homyk and Emerson, 1988, Deak et al., 1982)

up^int-3

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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