FlyBase Allele Report: Dmel\wapl[2]

General Information

Symbol

Dmel\wapl²

Species

D. melanogaster

Name

FlyBase ID

FBal0018419

Feature type

allele

Associated gene

Dmel\wapl

Associated Insertion(s)

Carried in Construct

Also Known As

wapl^C204, l(1)C204, C204

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

X ray

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Cunningham et al., 2012)

Mutagen

X ray

(Gandhi et al., 1992)

Progenitor genotype

Cytology

Description

970bp insertion of DNA from the Egfr gene into an exon of wapl that is common to both the large and small proteins eliminates the production of both protein isoforms.

(Cunningham et al., 2012)

Insertion of approximately 1.6kb DNA into wapl.

(Verni et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryo | germline clone | maternal effect | pre-blastoderm stage

(Verni et al., 2000)

embryo | germline clone | pre-blastoderm stage

(Perrimon et al., 1989)

heterochromatin | maternal effect

(Verni et al., 2000)

metaphase & condensed nuclear chromosome | maternal effect

(Verni et al., 2000)

Detailed Description

Statement

Reference

Mutants show a significant amount of chromatid stretching during mitotic anaphase compared to wild type.

(Oliveira et al., 2014)

Embryos derived from germline clones usually arrest prior to blastoderm formation but manage to form small patches of cuticle. Brain cells of mutant larvae show defects in heterochromatin organisation. In metaphase figures, the sister chromatids of the major autosomes and of the X chromosome are aligned parallel to each other, instead of the wild-type X- and V- shaped arrangements. The Y and 4th chromosomes are resolved into two distinct sister chromatids, in contrast to wild type. The sister chromatids of all mutant chromosomes are not completely separated but are instead connected by a thread located in the centromeric region. The mitotic index is normal. wapl²/In(1)FM7 females show a significant increase in X chromosome nondisjunction compared to control +/In(1)FM7 females. wapl²/+ females show the same level of X chromosome nondisjunction as control +/+ females.

(Verni et al., 2000)

Transmission rate of Dp(1;f)J21A through females to progeny is 28%, wapl mutation has no effect on transmission.

(Cook et al., 1997)

Lethality occurs during larval and pupal stages. Phenotype of homozygous germ line clones is maternal effect lethal.

(Perrimon et al., 1985)

L3 larval/pupal boundary lethal exhibit small disk phenotype; homozygous germ-line clones produce zygotes that arrest in preblastoderm stage.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT suppressed by

Statement

Reference

wapl² has phenotype, non-suppressible by Cyp4d1^unspecified

(Gandhi et al., 1992)

Additional Comments

Genetic Interactions

Statement

Reference

Mutant phenotype cannot be rescued by P element mediated transformation of a wild type copy of Cyp4d1.

(Gandhi et al., 1992)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

wapl² is rescued by wapl^GC

(Verni et al., 2000)

Not rescued by

wapl² is not rescued by Scer\GAL4^arm.PS/wapl^UAS.S

(Cunningham et al., 2012)

wapl² is not rescued by wapl^UAS.L/Scer\GAL4^arm.PS

(Cunningham et al., 2012)

wapl² is not rescued by wapl^tB

(Verni et al., 2000)

wapl² is not rescued by wapl^tA

(Verni et al., 2000)

Comments

The lethality of wapl² is not rescued by expression of either wapl^Scer\UAS.S or wapl^Scer\UAS.L under the control of Scer\GAL4^arm.PS.

(Cunningham et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (2)

5741

108397

Notes on Origin

Discoverer

Lefevre.

Comments

Maternal germline clonal analysis demonstrates there is a maternal effect lethal phenotype, preblastoderm defect.

(Perrimon et al., 1989)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

C204

(Perrimon et al., 1989, Kramers et al., 1983)

l(1)C204

(Gandhi et al., 1992, Perrimon et al., 1985)

pasc^C204

(Cook et al., 1997)

wapl²

(Cunningham et al., 2012, Gause et al., 2010, Popodi, 2010.10.8)

wapl^C204

(Oliveira et al., 2014, Eichinger et al., 2013, Verni et al., 2000)

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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