mus206A1 mutants have an increased frequency of single-strand annealing repair (SSA) compared to controls in a P{wIw.FRT} hemizygous assay to study DNA double-stranded break repair when assayed at 32oC or 38oC.
mus206A1 mutants have an increased SSA frequency compared to controls in a P{wIw.FRT}/P{wIw.FRT.yellow} homozygous assay to study DNA double-stranded break repair when assayed at 38oC, while the frequency of interhomolog gene conversion (GC) is unchanged.
Homozygous adults show a 7.5-fold increase in induced mutability compared to wild-type following treatment with methyl methanesulfonate, and a 2.9-fold increase in induced mutability compared to wild-type following treatment with ethyl methanesulfonate.
Hypermutability to alkylating agents; defective in alkylation repair pathway. Fecundity of homozygous females normal.
Homozygous larval brain ganglia have a reduced ability to synthesise DNA after exposure to N-acetoxy-2-acetylaminofluorene compared to wild-type. Larval survival hypersensitive to exposure to methyl methanesulfonate, nitrogen mustard and ultraviolet light, but not to formaldehyde or X rays. Partially deficient in postreplication repair.
Homozygous larvae are sensitive to UV light, nitrogen mustard and methyl methanesulfonate, but not to X rays or formaldehyde.
Recovered as a methyl methanesulfonate sensitive mutant.
Nucleotide incorporation is not increased in homozygous embryonic cells in culture after treatment with methyl methanesulfonate in an unscheduled DNA synthesis assay, indicating that the mutant flies are nearly completely deficient in alkylation-induced excision-repair function.