Hemizygous adults show a reduced response to propionic acid, ethyl butyrate, 2-butanone and ethyl acetate in an electroantennogram assay compared to control flies. The response to butanol, 1-octanol, benzaldehyde, butyl acetate and 2-heptanone in this assay is not significantly different from the response of control flies to these odorants. Mutant flies have fewer antennal neurons that are sensitive to ethyl butyrate than control flies. The proportion of antennal olfactory neurons that are responsive to membrane-permeant cyclic nucleotide analogues (8-Br-cAMP and 8-Br-cGMP) is also reduced in mutant flies compared to wild-type.

Response of the channel to modulation by protein kinases depends on temperature: I_A is increased at 5^oC but remains almost unchanged at 16^oC, conversely I_K is nearly normal at 5^oC and decreased at 16^oC. At 16^oC I_A and I_K levels, and I_CF and I_CS levels can be reduced by application of W7, a Ca²⁺/CaM antagonist. cGMP-modulation of I_K and I_A by the mutant channel by 8-Br-cGMP is altered.

Ether induced leg shaking and electrophysiological defect characteristic of eag mutations.

Severe defects in all K⁺ currents within larval muscle.