FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Act88FG368E
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General Information
Symbol
Dmel\Act88FG368E
Species
D. melanogaster
Name
FlyBase ID
FBal0028746
Feature type
allele
Associated gene
Dmel\Act88F
Associated Insertion(s)
Carried in Construct
P{Act88F-G368E}
Key Links
Transgenic product class
dominant_negative_variant
(Drummond et al., 1991)
missense_variant
(Drummond et al., 1991)
Mutagen
Nature of the Allele
Transgenic product class
dominant_negative_variant
(Drummond et al., 1991)
missense_variant
(Drummond et al., 1991)
Mutagen
in vitro construct
(Drummond et al., 1991, Drummond et al., 1990)
Progenitor genotype
Carried in construct
P{Act88F-G368E}
Cytology
Description

4kb genomic fragment encompassing the Act88F gene (includes approximately 1420bp of 5' flanking sequence and approximately 400bp of 3' flanking sequence). A G368E amino acid replacement has been introduced into the coding sequence.

(Drummond et al., 1991)
Allele components
Component
Use(s)
Encoded product / tool
Act88F
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Act88F6 null flies homozygous for Act88FG368E insert flew no better than the original null strain, flies carrying one mutant copy had almost normal flight ability. Indirect flight muscles in Act88F6 homozygous for Act88FG368E insert have only a slight disruption in structure.

      (Drummond et al., 1991)

      IFM myofibrils are studied in transformant Act88F6 mutants. Mutants permit the assembly of cross striated myofibrils.

      (Sparrow et al., 1991)

      LM study reveals a slight change in muscle ultrastructure. Flies had wild type levels of actin and could fly but less well than wild type: wing beat frequency was reduced by 20%. The amino acid change has significantly affected the kinetics of force generation by muscle fibres by affecting the binding of tropomyosin and the light chain of myosin to actin.

      (Drummond et al., 1990)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      Act88FG368E partially rescues Act88F6

      (Drummond et al., 1991)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Act88FG368E
      Name Synonyms
      Secondary FlyBase IDs
        References (6)