FlyBase Allele Report: Dmel\pie[EB3]

General Information

Symbol

Dmel\pie^EB3

Species

D. melanogaster

Name

FlyBase ID

FBal0032439

Feature type

allele

Associated gene

Dmel\pie

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Baker et al., 1992)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q391term.

(Shi et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:10,421,406..10,421,406 [-]

Nucleotide change:

C10421406T

Reported nucleotide change:

C?T

Amino acid change:

Q391term | pie-PA; Q391term | pie-PB

Reported amino acid change:

Q391term

(Shi et al., 2003)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate (with pie^E1-16)

(Shi et al., 2003)

abnormal developmental rate (with pie^G2-4)

(Shi et al., 2003)

abnormal developmental rate | recessive

(Shi et al., 2003)

female sterile (with Df(2L)J2)

(Shi et al., 2003)

increased cell death | recessive

(Shi et al., 2003)

increased cell death | somatic clone

(Shi et al., 2003)

male semi-sterile (with Df(2L)J2)

(Shi et al., 2003)

partially lethal - majority die (with Df(2L)J2)

(Shi et al., 2003)

partially lethal - majority live | recessive

(Shi et al., 2003)

sterile (with pie^E1-16)

(Shi et al., 2003)

sterile (with pie^G2-4)

(Shi et al., 2003)

sterile (with pie^QM47)

(Shi et al., 2003)

viable

(Bach et al., 2003)

visible (with pie^E1-16)

(Xing et al., 2015)

visible | recessive

(Baker et al., 1992)

Phenotype Manifest In

cone cell

(Shi et al., 2003)

eye

(Shi et al., 2003, Baker et al., 1992)

eye (with pie^E1-2)

(Shi et al., 2003)

eye (with pie^E1-16)

(Xing et al., 2015, Shi et al., 2003)

eye (with pie^G2-4)

(Shi et al., 2003)

eye (with pie^H113)

(Shi et al., 2003)

eye (with pie^QM47)

(Shi et al., 2003)

eye disc

(Baker et al., 1992)

eye photoreceptor cell

(Shi et al., 2003)

leg disc

(Shi et al., 2003)

ommatidium

(Shi et al., 2003, Baker et al., 1992)

ommatidium (with pie^E1-2)

(Shi et al., 2003)

ommatidium (with pie^E1-16)

(Shi et al., 2003)

ommatidium (with pie^G2-4)

(Shi et al., 2003)

ommatidium (with pie^H113)

(Shi et al., 2003)

ommatidium (with pie^QM47)

(Shi et al., 2003)

pigment cell

(Shi et al., 2003)

wing

(Baker et al., 1992)

wing disc

(Shi et al., 2003)

Detailed Description

Statement

Reference

pie^E1-16/pie^EB3 mutant adult survivors exhibit a marked rough eye phenotype.

(Xing et al., 2015)

Homozygous pie^EB3 flies have rough eyes. Facet size varies from smaller than normal to enlarged (possible fused) facets. Cells of all types (pigment, cone, photoreceptor) appear to be randomly missing from ommatidial arrays. The second mitotic wave proceeds normally in pie^EB3 homozygous eye discs, but unlike wild-type abundant apoptotic bodies are evident both anterior and posterior to the morphogenetic furrow. Excess cell death is also seen in wing and leg imaginal discs in these flies. On average pie^EB3 homozygous adult emerge 2.5 days later than their pie^EB3/+ siblings. Homozygotes comprise 23% of the adult population instead of the expected 50%. pie^EB3 is semi-lethal over Df(2L)J2, Df(2L)J27, Df(2L)J39, Df(2L)J106 or Df(2L)J77. Those surviving to adulthood are very sickly, females are infertile, or nearly so, and males have reduced fertility. Somatic clones of pie^EB3 cells in the adult eye have almost normal morphology. However, pie^EB3 somatic clones in the eye disc have increased cell death.

(Shi et al., 2003)

Most ommatidia contain normal array of retinula cells, photoreceptor cells are sometimes directly adjacent to neighbouring units. Associated sets of photoreceptor cells occupy a single enlarged facet and are jointly surrounded by pigment cells. Homozygotes have heldout notched wings. Eye discs sometimes show irregular spacing of the ommatidia in homozygotes. Homozygous adults have a rough eye phenotype.

(Baker et al., 1992)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

pie^EB3 has increased cell death | recessive phenotype, suppressible by BacA\p35^GMR.PH

(Shi et al., 2003)

Suppressor of

Statement

Reference

pie^EB3 is a suppressor of visible phenotype of upd1^GMR.PB

(Bach et al., 2003)

Other

Statement

Reference

hid^unspecified, pie^EB3/pie[+] has lethal | dominant phenotype

(Shi et al., 2003)

W[+]/hid^unspecified, pie^EB3 has lethal | dominant phenotype

(Shi et al., 2003)

Phenotype Manifest In

Suppressed by

Statement

Reference

pie^EB3 has eye phenotype, suppressible by BacA\p35^GMR.PH

(Shi et al., 2003)

Suppressor of

Statement

Reference

pie^EB3 is a suppressor of eye phenotype of upd1^GMR.PB

(Bach et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

In pie^EB3 homozygotes the cell death increase posterior to the morphogenetic furrow in eye discs is suppressed and the rough eye phenotype of adults is mostly suppressed by BacA\p35^GMR.PH. pie^EB3/+ is lethal in combination with W^unspecified/+.

(Shi et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

3792

pie^EB3/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

pie^EB3

(Xing et al., 2015, Shi et al., 2003)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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