FB2026_02 , released June 18, 2026
Allele: Dmel\eEF1α1hs.PS
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General Information
Symbol
Dmel\eEF1α1hs.PS
Species
D. melanogaster
Name
heat shock construct of Shepherd
FlyBase ID
FBal0033659
Feature type
allele
Associated gene
Dmel\eEF1α1
Associated Insertion(s)
Carried in Construct
P{hsp70-EF-1α}
Key Links
Transgenic product class
wild_type
(Shepherd et al., 1989)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Shepherd et al., 1989)
Mutagen
in vitro construct
(Stearns and Kaiser, 1996, Shikama et al., 1994, Shepherd et al., 1989)
Progenitor genotype
Carried in construct
P{hsp70-EF-1α}
Cytology
Description

An Hsp70 promoter drives expression of eEF1α1.

(Shepherd et al., 1989)
Allele components
Component
Use(s)
Regulatory region(s)
Hsp70
Encoded product / tool
eEF1α1
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      In lines E1, E2, E3 and E4 expression is detected at 37oC. Lines E5 and E6 have no detectable expression. These lines and control lines differed mainly in the size and position of the insert. Effects of position and effects of interaction of inserts with the genetic background have significant effects on mated female and virgin male lifespan at 29 and 37oC, individuals carrying the insertions live significantly longer. Effects of insert size are smaller but significant.

      (Kaiser et al., 1997)

      Flies carrying the P{hsp70-EF-1α} construct live longer at 29oC than control flies carrying the same construct without the Ef1α48D gene.

      (Shikama et al., 1994)

      Females with enhanced expression of Ef1α48D live significantly longer and laid significantly fewer eggs early in life than do control females. This is true at both 25oC and 29oC. Enhanced expression has no impact on the life span of virgin males.

      (Stearns et al., 1993)

      At 25[o]C or 29[o]C transformant flies have a longer lifespan than nontransformed flies depending on the P-element insertion site.

      (Shepherd et al., 1989)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer

      Shepherd.

      (Shikama et al., 1994)
      Comments
      Comments

      Flies carrying Ef1α48Dhs.PS have been used in a study of the impact of P-elements on fitness components in Drosophila.

      (Stearns and Kaiser, 1996)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Ef1α48Dhs.PS
      eEF1α1hs.PS
      Name Synonyms
      heat shock construct of Shepherd
      (Shepherd et al., 1989)
      Secondary FlyBase IDs
        References (5)