FB2026_02 , released June 18, 2026
Allele: Dmel\Rophs.PS
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General Information
Symbol
Dmel\Rophs.PS
Species
D. melanogaster
Name
heat shock construct of Schulze
FlyBase ID
FBal0033988
Feature type
allele
Associated gene
Dmel\Rop
Associated Insertion(s)
Carried in Construct
P{hsrop}
Key Links
Transgenic product class
wild_type
(Schulze et al., 1994)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Schulze et al., 1994)
Mutagen
in vitro construct
(Wu et al., 1998, Schulze et al., 1994)
Progenitor genotype
Carried in construct
P{hsrop}
Cytology
Description

Expression of a Rop partial cDNA containing the 5' leader and the entire ORF and a genomic fragment that encompasses the 3' untranslated region is driven by an Hsp70 promoter, influenced by Hsp70 3' sequences.

(Schulze et al., 1994)
Allele components
Component
Use(s)
Regulatory region(s)
Hsp70
Encoded product / tool
Rop
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Overexpression of Rophs.PS using heat shock causes a 45% decrease in the amplitude of the excitatory junctional potential (EJP) in the muscle at the larval neuromuscular junction compared to control larvae. This phenotype is not altered by coexpression of Snap25hs.PW. Synapses at the neuromuscular junction of heat shocked larvae carrying Rophs.PS appear normal. Overexpression of both Rophs.PS and Syx1Ahs.PW using heat shock partially suppresses the physiological defects seen in the muscle at the larval neuromuscular junction when either is overexpressed alone.

      (Wu et al., 1998)

      Heat shocked larvae exhibit a 45-35% decrease in the amplitude of evoked neurotransmitter release. Heterozygotes exhibit significantly less reduction in neurotransmitter release compared with homozygous larvae. Synaptic responses in larvae allowed to recover after heat shock are pre-heat shock levels. There is no obvious change in the synaptic vesicle pool that can be detected at the light microscope level.

      (Schulze et al., 1994)

      Expressed in Schneider cells to identify the gene product.

      (Salzberg et al., 1993)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Rophs.PS
      Name Synonyms
      heat shock construct of Salzberg
      (Salzberg et al., 1993)
      heat shock construct of Schulze
      Secondary FlyBase IDs
        References (3)